Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR

One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and t...

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Bibliographic Details
Published in:Systematic and applied microbiology 2003-06, Vol.26 (2), p.254-261
Main Authors: Gaviria Rivera, Adelaida M., Priest, Fergus G.
Format: Article
Language:English
Subjects:
Bacillus thuringiensis
Bacillus thuringiensis - classification
Bacillus thuringiensis - genetics
Bacterial Typing Techniques - methods
Biological and medical sciences
clone
DNA, Bacterial - genetics
Epidemiology
Fundamental and applied biological sciences. Psychology
Genome, Bacterial
Microbiology
Mycology
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
RAPD
Serotyping
serovar
Species Specificity
Virology
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Summary:One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and tolworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars aizawai, canadensis, entomocidus and sotto biotype dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.
ISSN:0723-2020
1618-0984
DOI:10.1078/072320203322346100