Molecular typing and antifungal susceptibility of clinical and environmental Cryptococcus neoformans species complex isolates in Goiania, Brazil

Summary A total of 124 Cryptococcus isolates, including 84 clinical strains obtained from cerebrospinal fluid from AIDS patients and 40 environmental isolates from pigeon excreta and from Eucalyptus trees, were studied. The varieties, serotypes, phospholipase activity and molecular profile of these...

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Bibliographic Details
Published in:Mycoses 2010-01, Vol.53 (1), p.62-67
Main Authors: Souza, L. K. H., Souza Junior, A. H., Costa, C. R., Faganello, J., Vainstein, M. H., Chagas, A. L. B., Souza, A. C. M., Silva, M. R. R.
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - pharmacology
biotyping
Brazil
Columbidae - microbiology
Cryptococcosis - microbiology
Cryptococcus gattii - classification
Cryptococcus gattii - drug effects
Cryptococcus gattii - genetics
Cryptococcus gattii - isolation & purification
Cryptococcus neoformans
Cryptococcus neoformans - classification
Cryptococcus neoformans - drug effects
Cryptococcus neoformans - genetics
Cryptococcus neoformans - isolation & purification
DNA Fingerprinting - methods
Environmental Microbiology
Eucalyptus
Eucalyptus - microbiology
Feces - microbiology
Genotype
Humans
Microbial Sensitivity Tests
molecular typing
Mycological Typing Techniques - methods
Phospholipases - metabolism
Serotyping
susceptibility
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Summary:Summary A total of 124 Cryptococcus isolates, including 84 clinical strains obtained from cerebrospinal fluid from AIDS patients and 40 environmental isolates from pigeon excreta and from Eucalyptus trees, were studied. The varieties, serotypes, phospholipase activity and molecular profile of these isolates were determined. Cryptococcus neoformans var. grubii serotype A was identified in 120 isolates and Cryptococcus gattii serotype B in four isolates. The clinical isolates showed higher phospholipase activity than environmental isolates. Similar patterns of in vitro susceptibility to amphotericin B, fluconazole, itraconazole and voriconazole and no resistance were found for all isolates. Molecular type VNI (C. neoformans var. grubii) was recovered in 80 clinical and 40 environmental isolates while the type VGIII (C. gattii) was found in four clinical isolates. This study demonstrated for the first time the molecular types of clinical and environmental Cryptococcus isolates in the midwest Brazil region.
ISSN:0933-7407
1439-0507
DOI:10.1111/j.1439-0507.2008.01662.x