Expression of caveolin-1 in human adipose tissue is upregulated in obesity and obesity-associated type 2 diabetes mellitus and related to inflammation

• Published in: Clinical endocrinology (Oxford) 2008-02, Vol.68 (2), p.213-219
• Main Authors: Catalán, Victoria, Gómez-Ambrosi, Javier, Rodríguez, Amaia, Silva, Camilo, Rotellar, Fernando, Gil, María J., Cienfuegos, Javier A., Salvador, Javier, Frühbeck, Gema
• Format: Article
• Language: English
• Subjects: Adipose Tissue - metabolism; Adult; Biological and medical sciences; Caveolin 1 - genetics; Caveolin 1 - metabolism; Diabetes Mellitus, Type 2 - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Female; Fundamental and applied biological sciences. Psychology; Humans; Inflammation - genetics; Inflammation - metabolism; Intra-Abdominal Fat - metabolism; Medical sciences; Metabolic diseases; Obesity; Obesity - genetics; Obesity - metabolism; Real-Time Polymerase Chain Reaction; Subcutaneous Fat - metabolism; Vertebrates: endocrinology
• ISSN: 0300-0664; 1365-2265
• DOI: 10.1111/j.1365-2265.2007.03021.x

Summary Objective  Caveolin‐1 (CAV‐1) plays important roles in many aspects of cellular biology, including vesicular transport, cholesterol homeostasis and signal transduction. The aim of the present study was to explore gene expression levels of CAV‐1 in human adipose tissue in obesity and obesity‐associated type 2 diabetes mellitus (T2DM) and to analyse its potential implication in the inflammatory state associated with obesity. Design and methods  Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) obtained from 15 females were used in the study. Patients were classified as lean (BMI 20·8 ± 1·0 kg/m2) or obese (BMI 50·5 ± 2·6 kg/m2). The obese group was further subclassified as normoglycaemic (NG) or patients with T2DM. Anthropometric measurements as well as circulating metabolites, hormones and adipokines were determined. Real‐time polymerase chain reaction (PCR) analyses were performed to quantify transcript levels of CAV‐1 and monocyte chemoattractant protein (MCP‐1). Results  The presence of CAV‐1 protein was detected in VAT and SAT by immunohistochemistry. Both obese NG and with T2DM patients exhibited significantly higher CAV‐1 expression levels in VAT and SAT compared with lean subjects (P