Tamoxifen epigenetically modulates CXCL12 expression in MCF-7 breast cancer cells

Abstract The CXCL12 chemokine binds to the CXCR4 receptor and contributes to survival, proliferation, and migration of malignant cells. Recent reports indicate that breast cancer cells lacking expression of CXCL12 but exhibiting CXCR4 can metastasize to target organs that secrete CXCL12. We observed...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2010-01, Vol.64 (1), p.54-57
Main Authors: Pietkiewicz, P.P, Lutkowska, A, Lianeri, M, Jagodzinski, P.P
Format: Article
Language:English
Subjects:
Antimetabolites, Antineoplastic - pharmacology
Antineoplastic Agents, Hormonal - pharmacology
Azacitidine - analogs & derivatives
Azacitidine - pharmacology
Biological and medical sciences
Breast cancer
Breast Neoplasms - drug therapy
Breast Neoplasms - genetics
Cell Line, Tumor
Chemokine CXCL12 - drug effects
Chemokine CXCL12 - genetics
CXCL12
Cytosine - metabolism
DNA Methylation - drug effects
Epigenesis, Genetic - drug effects
Female
Gene Expression Regulation, Neoplastic - drug effects
Gynecology. Andrology. Obstetrics
Humans
Internal Medicine
Mammary gland diseases
Medical Education
Medical sciences
Pharmacology. Drug treatments
Sequence Analysis, DNA - methods
Tamoxifen
Tamoxifen - pharmacology
Tumors
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Summary:Abstract The CXCL12 chemokine binds to the CXCR4 receptor and contributes to survival, proliferation, and migration of malignant cells. Recent reports indicate that breast cancer cells lacking expression of CXCL12 but exhibiting CXCR4 can metastasize to target organs that secrete CXCL12. We observed that Tamoxifen (Tam), similarly to 5-dAzaC, results in significantly increased levels of CXCL12 transcript and protein in MCF-7 breast cancer cells. Bisulfite sequencing suggests that Tam, similarly to 5-dAzaC, may increase CXCL12 expression via reduction in methylation of cytosine in the cytosine-guanosine (CpG) dinucleotide island of the CXCL12 promoter of MCF-7 cells. Our results, together with findings of other researches, may suggest that Tam epigenetically activates CXCL12 expression in breast cancer cells and can make these cells less susceptible to attraction by exogenous CXCL12 to metastasis sites.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2009.04.041