Mechanisms of sevoflurane-induced myocardial preconditioning in isolated human right atria in vitro

• Published in: Anesthesiology (Philadelphia) 2003-07, Vol.99 (1), p.27-33
• Main Authors: YVON, Alexandra, HANOUZ, Jean-Luc, HAELEWYN, Benoit, TERRIEN, Xavier, MASSETTI, Massimo, BABATASI, Gérard, KHAYAT, André, DUCOURET, Pierre, BRICARD, Henri, GERARD, Jean-Louis
• Format: Article
• Language: English
• Subjects: Aged; Anesthetics, Inhalation - pharmacology; ATP-Binding Cassette Transporters; Benzamides - pharmacology; Biological and medical sciences; Cardiovascular system; Decanoic Acids - pharmacology; Heart Atria - drug effects; Humans; Hydroxy Acids - pharmacology; Hypoxia - physiopathology; In Vitro Techniques; Ischemic Preconditioning, Myocardial; Isometric Contraction - drug effects; KATP Channels; Medical sciences; Methyl Ethers - pharmacology; Middle Aged; Miscellaneous; Myocardial Contraction - drug effects; Pharmacology. Drug treatments; Potassium Channel Blockers - pharmacology; Potassium Channels - drug effects; Potassium Channels - physiology; Potassium Channels, Inwardly Rectifying; Purinergic P1 Receptor Antagonists; Receptors, Purinergic P1 - physiology; Reperfusion Injury - physiopathology; Sarcolemma - metabolism; Sevoflurane; Xanthines - pharmacology
• ISSN: 0003-3022; 1528-1175
• DOI: 10.1097/00000542-200307000-00008

The authors examined the role of adenosine triphosphate-sensitive potassium channels and adenosine A(1) receptors in sevoflurane-induced preconditioning on isolated human myocardium. The authors recorded isometric contraction of human right atrial trabeculae suspended in oxygenated Tyrode's solution (34 degrees C; stimulation frequency, 1 Hz). In all groups, a 30-min hypoxic period was followed by 60 min of reoxygenation. Seven minutes before hypoxia reoxygenation, muscles were exposed to 4 min of hypoxia and 7 min of reoxygenation or 15 min of sevoflurane at concentrations of 1, 2, and 3%. In separate groups, sevoflurane 2% was administered in the presence of 10 microm HMR 1098, a sarcolemmal adenosine triphosphate-sensitive potassium channel antagonist; 800 microm 5-hydroxy-decanoate, a mitochondrial adenosine triphosphate-sensitive potassium channel antagonist; and 100 nm 8-cyclopentyl-1,3-dipropylxanthine, an adenosine A(1) receptor antagonist. Recovery of force at the end of the 60-min reoxygenation period was compared between groups (mean +/- SD). Hypoxic preconditioning (90 +/- 4% of baseline) and sevoflurane 1% (82 +/- 3% of baseline), 2% (92 +/- 5% of baseline), and 3% (85 +/- 7% of baseline) enhanced the recovery of force after 60 min of reoxygenation compared with the control groups (52 +/- 9% of baseline). This effect was abolished in the presence of 5-hydroxy-decanoate (55 +/- 14% of baseline) and 8-cyclopentyl-1,3-dipropylxanthine (58 +/- 16% of baseline) but was attenuated in the presence of HMR 1098 (73 +/- 10% of baseline). In vitro, sevoflurane preconditions human myocardium against hypoxia through activation of adenosine triphosphate-sensitive potassium channels and stimulation of adenosine A(1) receptors.