Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmici...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience research 2010-02, Vol.66 (2), p.223-227
Main Authors: Ebisawa, Takashi, Numazawa, Kahori, Shimada, Hiroko, Izutsu, Hiroyuki, Sasaki, Tsukasa, Kato, Nobumasa, Tokunaga, Katsushi, Mori, Akio, Honma, Ken-ichi, Honma, Sato, Shibata, Shigenobu
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Circadian Rhythm - genetics
Clock genes
CLOCK Proteins - genetics
Gene Expression
Human biological clock
Human circadian rhythm
Humans
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - physiology
Luciferase
Male
Mice
Mononuclear cells
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Self-sustained circadian rhythm
Sleep Wake Disorders - genetics
Sleep Wake Disorders - physiopathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus—the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.
ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2009.10.007