Pitfalls in the assay of carboxymethylcellulase activity

A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1983-02, Vol.25 (2), p.377-385
Main Authors: Lindner, W. A., Dennison, C., Quicke, G. V.
Format: Article
Language:English
Subjects:
assays
carboxymethylcellulose
cellulase
Sclerotium rolfsii
Trichoderma reesei
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Summary:A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature of both the enzymes and the substrate make the assay unsuitable for use in the calculation of enzyme recovery and purity.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260250207