Covalent coupling of microorganisms to a cellulosic support

Methods for the covalent coupling of microorganisms to a solid support were investigated. Both bacteria and yeast were attached to cellulose particles using cyanuric chloride as the coupling agent, although different experimental procedures were needed for the two types of microbes. This general tec...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1985-05, Vol.27 (5), p.632-637
Main Authors: Okita, W. Blair, Bonham, D. Bivings, Gainer, John L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Immobilization of organelles and whole cells
Immobilization techniques
Methods. Procedures. Technologies
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Summary:Methods for the covalent coupling of microorganisms to a solid support were investigated. Both bacteria and yeast were attached to cellulose particles using cyanuric chloride as the coupling agent, although different experimental procedures were needed for the two types of microbes. This general technique for whole‐cell immobilization offers an advantage over entrapment methods in that the cells are attached to the outer surface of the solid, thus eliminating the resistance of a gel to the transfer of nutrients and products. There are also indications that such immobilized cells show high productivities.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260270513