Comparisons between cellulase production by Aspergillus fumigatus in agitated vessels and in an air-lift fermentor

Aspergillus fumigatus was cultured in disc‐turbine‐agitated vessels and in an air‐lift fermentor. In the agitated vessels the yield of cellulase was reduced when the agitation rate was increased, although extracellular protein levels rose. The enzyme complex itself was shown to be exceptionally stab...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1985-08, Vol.27 (8), p.1166-1172
Main Authors: Wase, D.A.J, McManamey, W.J, Raymahasay, S, Vaid, A.K
Format: Article
Language:English
Subjects:
aeration
ASPERGILLUS
Aspergillus fumigatus
Biological and medical sciences
Biotechnology
CELLULASE
CELULASA
Enzyme engineering
FERMENTACION
FERMENTATION
fermenters
Food industries
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
PRODUCCION
PRODUCTION
Production of selected enzymes
Use and upgrading of agricultural and food by-products. Biotechnology
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Summary:Aspergillus fumigatus was cultured in disc‐turbine‐agitated vessels and in an air‐lift fermentor. In the agitated vessels the yield of cellulase was reduced when the agitation rate was increased, although extracellular protein levels rose. The enzyme complex itself was shown to be exceptionally stable under conditions similar to those in the agitated vessels, so probably shear damage to the mycelium had occurred, liberating intracellular contents. These appeared to contain an inhibitor that could be removed by fabricated inorganic protein absorbents, such as kieselguhr and alumina. However, the inhibitor was not likely to be protease, since only relatively low levels could be detected and its identity has not been established. The use of an air‐lift fermentor avoided the shear effects due to use of the disc turbine agitator in the conventional fermentors, and yields of enzyme were then found to increase by about 20%, maximum yields being obtained at maximum KLa values.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260270811