The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley (Hordeum vulgare L.) uninucleate microspores

The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with...

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Bibliographic Details
Published in:Plant cell reports 2003-07, Vol.21 (11), p.1065-1071
Main Authors: SHIM, Y. S, KASHA, K. J
Format: Article
Language:English
Subjects:
Barley
Biological and medical sciences
Cell cycle
Cell Cycle - drug effects
Cell Nucleus - drug effects
Cell Nucleus - genetics
Cold Temperature
Cultivars
Deoxyribonucleic acid
DNA
DNA Replication - drug effects
DNA, Plant - analysis
DNA, Plant - biosynthesis
Fundamental and applied biological sciences. Psychology
Haploidy
Hordeum - cytology
Hordeum - drug effects
Hordeum - genetics
Mannitol - pharmacology
Transformation, Genetic
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Summary:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in early, mid, and late uninucleate stages of the two-rowed barley cultivars Manley and Igri were subjected to two commonly used pretreatments. First, during cold pretreatment for 28 days there was a slow increase in relative DNA values as well as asymmetric nuclear divisions in some microspores. Second, during a 4-day cold plus 0.3 M mannitol pretreatment, there was very little change in the microspore stage or DNA values indicating that for the duration of this pretreatment the progression of the cell cycle was essentially suspended at all stages, both in Igri and Manley. The results are discussed relative to the potential for genetic transformation of microspores.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-003-0635-4