Differential effects of Matrigel and its components on functional activity of CFTR and ENaC in mouse endometrial epithelial cells

• Published in: Cell biology international 2003-07, Vol.27 (7), p.543-548
• Main Authors: Yang, Jian Zhi, Ho, Alice Lok Sze, Ajonuma, Louis Chukwuemeka, Lam, Sun Yee, Tsang, Lai Ling, Tang, Ning, Rowlands, Dewi Kenneth, Gou, Yu Lin, Chung, Yiu Wa, Chan, Hsiao Chang
• Format: Article
• Language: English
• Subjects: Animals; Cells, Cultured; CFTR and ENaC; Collagen; Collagen - pharmacology; Cystic Fibrosis Transmembrane Conductance Regulator - metabolism; Drug Combinations; Endometrium - drug effects; Endometrium - metabolism; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Sodium Channels; Female; Laminin; Laminin - pharmacology; Matrigel; Mice; Mice, Inbred ICR; Proteoglycans - pharmacology; Short circuit current; Sodium Channels - metabolism
• ISSN: 1065-6995; 1095-8355
• DOI: 10.1016/S1065-6995(03)00079-9

Our previous studies have observed an effect of Matrigel, a solubilized basement membrane preparation extracted from the Engelbreth–Holm–Swarm (EHS) mouse sarcoma, on the expression of ion channels in mouse endometrial epithelia; namely the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent Cl −channel, and the epithelial sodium channel (ENaC). The present study further investigated the effects of Matrigel and its individual components on the functional expression of CFTR and ENaC using the short-circuit current (Isc) technique. The results showed that different components of Matrigel, namely growth factors, laminin and collagen, had differential effects on the functional activity of the two ion channels in murine endometrial epithelium. The information obtained may be useful for designing future in vitro culture models to investigate the functional roles of these ion channels in the endometrium.