Simultaneous analysis of six microsatellite markers in Atlantic cod ( Gadus morhua): a novel multiplex assay system for use in selective breeding studies

A novel hexaplex assay system including Gmo8, Gmo19, Gmo35, Gmo37, Tch11, and Tch12 microsatellites from Atlantic cod, consisting of trinucleotide or tetranucleotide repeat units, is introduced. All 6 loci were coamplified in a single reaction employing dye-labeled primers. Alleles from these loci w...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2003-04, Vol.5 (2), p.141-148
Main Authors: Delghandi, Madjid, Mortensen, Atle, Westgaard, Jon-Ivar
Format: Article
Language:English
Subjects:
Animal Husbandry
Animals
Base Sequence
Bioassays
Biological Assay - methods
DNA - isolation & purification
Fishes - genetics
Gadus morhua
Genetic markers
Genotypes
Marine
Marine biology
Microsatellite Repeats - genetics
Molecular Sequence Data
Pilot Projects
Polymorphism, Genetic - genetics
Reproducibility of Results
Selection, Genetic
Selective breeding
Studies
Tandem Repeat Sequences - genetics
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Summary:A novel hexaplex assay system including Gmo8, Gmo19, Gmo35, Gmo37, Tch11, and Tch12 microsatellites from Atlantic cod, consisting of trinucleotide or tetranucleotide repeat units, is introduced. All 6 loci were coamplified in a single reaction employing dye-labeled primers. Alleles from these loci were sized using an internal standard by automated sample processing in an ABI 310 Genetic Analyser. Amplified alleles in profiles containing selected microsatellites were typed clearly, providing easily interpretable results. Sequencing data indicated that alleles at all loci consisted of simple repeat units. This may help minimize the likelihood of stuttering upon polymerase chain reaction amplification. The results suggest that the presented hexaplex assay system may be a useful tool in a selective breeding program in which genetic identification will allow different genotypes to be reared together from fertilization. This should have a great impact as it will make selective breeding more efficient.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-002-0086-x