Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys

A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Ver...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical sciences 1992-12, Vol.81 (12), p.1178-1180
Main Authors: Bikhazi, Anwar B., Medlej‐Hashim, Myrna, El‐Kasti, Muna
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - metabolism
Calcium-Binding Proteins - metabolism
Chemistry
Chemistry, Medicinal
Chemistry, Multidisciplinary
Ethacrynic Acid - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Kidney - drug effects
Kidney - metabolism
Kinetics
Life Sciences & Biomedicine
Ouabain - pharmacology
Perfusion
Pharmacology & Pharmacy
Physical Sciences
Rats
Rats, Sprague-Dawley
Science & Technology
Sodium - physiology
Verapamil - pharmacology
Vertebrates: urinary system
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4560-3e0f6478184cb1b4ef86a44c4489a25ff87222a6c5cccfb941ad22edec09f96f3
cites
container_end_page 1180
container_issue 12
container_start_page 1178
container_title Journal of pharmaceutical sciences
container_volume 81
creator Bikhazi, Anwar B.
Medlej‐Hashim, Myrna
El‐Kasti, Muna
description A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Verapamil (1mM) in Krebs‐improved Ringer solution increased calcium retention in the kidneys by ~ 117% compared with controls. With Na‐free Krebs‐improved Ringer solution, calcium retention increased by only 92%. However, in Krebs‐improved Ringer solutions containing 59 and 122 mequivalents of Na, calcium retention in the kidney increased by 46 and 43%, respectively, compared with controls. With Krebs‐improved Ringer solution containing 15mM ouabain, calcium retention in the kidney decreased by 29.5%, whereas with 15mM ouabain plus 1mM ethacrynic acid in the perfusate, the effect on calcium retention in the kidney was insignificant compared with controls. These results suggest that two sodium‐dependent calcium‐transporting systems exist at the peritubular side of the kidney tubules: (1) a Na+–Ca+2 countertransport system sensitive to verapamil and (2) a Na+–Ca+2 cotransport system sensitive to the intracellular concentration of sodium.
doi_str_mv 10.1002/jps.2600811210
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73442081</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022354915490197</els_id><sourcerecordid>73442081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4560-3e0f6478184cb1b4ef86a44c4489a25ff87222a6c5cccfb941ad22edec09f96f3</originalsourceid><addsrcrecordid>eNqNks9v0zAUxy0EGmVw5YbkA9qFpdiO88PHKiqDrbBphSFxsRznWXgkTrEToP89rlK14oDgZMnv831672Mj9JySOSWEvb7fhDnLCSkpZZQ8QDOaMZLkhBYP0SwCLEkzLh6jJyHcE0JykmUn6IRyQdM0myG5NAb0EHBv8B14tVGdbc_x9ahqZd05Vq7By-Gr0n7rrMYLbRvcO1ypVtuxw7cwgBtsvLEO34A3Y4AG36oBX9nGwTY8RY-MagM825-n6NOb5cfqbbK6vnhXLVaJ5llOkhSIyXlR0pLrmtYcTJkrzjXnpVAsM6YsGGMq15nW2tSCU9UwBg1oIozITXqKzqa-G99_HyEMsrNBQ9sqB_0YZJFyzqKkCM4nUPs-BA9GbrztlN9KSuTOqIxG5dFoDLzYdx7rDpojPimM9Zf7ugpatcYrp204YDyL1sWuTTlhP6HuTdAWnIYDtaBCsKuFiAPs8MoOame16kc3xOir_49GWuxp28L2H9vJy5v1H7smU9aGAX4dssp_k3mRFpn8_OFCsi-ry_Vdupbvj0tBfNofFrzcT9dYH3-VbHr7N62_AX2p0Rw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73442081</pqid></control><display><type>article</type><title>Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys</title><source>Wiley Online Library Journals</source><creator>Bikhazi, Anwar B. ; Medlej‐Hashim, Myrna ; El‐Kasti, Muna</creator><creatorcontrib>Bikhazi, Anwar B. ; Medlej‐Hashim, Myrna ; El‐Kasti, Muna</creatorcontrib><description>A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Verapamil (1mM) in Krebs‐improved Ringer solution increased calcium retention in the kidneys by ~ 117% compared with controls. With Na‐free Krebs‐improved Ringer solution, calcium retention increased by only 92%. However, in Krebs‐improved Ringer solutions containing 59 and 122 mequivalents of Na, calcium retention in the kidney increased by 46 and 43%, respectively, compared with controls. With Krebs‐improved Ringer solution containing 15mM ouabain, calcium retention in the kidney decreased by 29.5%, whereas with 15mM ouabain plus 1mM ethacrynic acid in the perfusate, the effect on calcium retention in the kidney was insignificant compared with controls. These results suggest that two sodium‐dependent calcium‐transporting systems exist at the peritubular side of the kidney tubules: (1) a Na+–Ca+2 countertransport system sensitive to verapamil and (2) a Na+–Ca+2 cotransport system sensitive to the intracellular concentration of sodium.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600811210</identifier><identifier>PMID: 1491335</identifier><identifier>CODEN: JPMSAE</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Calcium-Binding Proteins - metabolism ; Chemistry ; Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Ethacrynic Acid - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Kidney - drug effects ; Kidney - metabolism ; Kinetics ; Life Sciences &amp; Biomedicine ; Ouabain - pharmacology ; Perfusion ; Pharmacology &amp; Pharmacy ; Physical Sciences ; Rats ; Rats, Sprague-Dawley ; Science &amp; Technology ; Sodium - physiology ; Verapamil - pharmacology ; Vertebrates: urinary system</subject><ispartof>Journal of pharmaceutical sciences, 1992-12, Vol.81 (12), p.1178-1180</ispartof><rights>1992 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>3</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wosA1992KA91000009</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c4560-3e0f6478184cb1b4ef86a44c4489a25ff87222a6c5cccfb941ad22edec09f96f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600811210$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600811210$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=4500090$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1491335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bikhazi, Anwar B.</creatorcontrib><creatorcontrib>Medlej‐Hashim, Myrna</creatorcontrib><creatorcontrib>El‐Kasti, Muna</creatorcontrib><title>Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys</title><title>Journal of pharmaceutical sciences</title><addtitle>J PHARM SCI</addtitle><addtitle>J. Pharm. Sci</addtitle><description>A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Verapamil (1mM) in Krebs‐improved Ringer solution increased calcium retention in the kidneys by ~ 117% compared with controls. With Na‐free Krebs‐improved Ringer solution, calcium retention increased by only 92%. However, in Krebs‐improved Ringer solutions containing 59 and 122 mequivalents of Na, calcium retention in the kidney increased by 46 and 43%, respectively, compared with controls. With Krebs‐improved Ringer solution containing 15mM ouabain, calcium retention in the kidney decreased by 29.5%, whereas with 15mM ouabain plus 1mM ethacrynic acid in the perfusate, the effect on calcium retention in the kidney was insignificant compared with controls. These results suggest that two sodium‐dependent calcium‐transporting systems exist at the peritubular side of the kidney tubules: (1) a Na+–Ca+2 countertransport system sensitive to verapamil and (2) a Na+–Ca+2 cotransport system sensitive to the intracellular concentration of sodium.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Chemistry</subject><subject>Chemistry, Medicinal</subject><subject>Chemistry, Multidisciplinary</subject><subject>Ethacrynic Acid - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kidney - drug effects</subject><subject>Kidney - metabolism</subject><subject>Kinetics</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Ouabain - pharmacology</subject><subject>Perfusion</subject><subject>Pharmacology &amp; Pharmacy</subject><subject>Physical Sciences</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Science &amp; Technology</subject><subject>Sodium - physiology</subject><subject>Verapamil - pharmacology</subject><subject>Vertebrates: urinary system</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EZCTM</sourceid><recordid>eNqNks9v0zAUxy0EGmVw5YbkA9qFpdiO88PHKiqDrbBphSFxsRznWXgkTrEToP89rlK14oDgZMnv831672Mj9JySOSWEvb7fhDnLCSkpZZQ8QDOaMZLkhBYP0SwCLEkzLh6jJyHcE0JykmUn6IRyQdM0myG5NAb0EHBv8B14tVGdbc_x9ahqZd05Vq7By-Gr0n7rrMYLbRvcO1ypVtuxw7cwgBtsvLEO34A3Y4AG36oBX9nGwTY8RY-MagM825-n6NOb5cfqbbK6vnhXLVaJ5llOkhSIyXlR0pLrmtYcTJkrzjXnpVAsM6YsGGMq15nW2tSCU9UwBg1oIozITXqKzqa-G99_HyEMsrNBQ9sqB_0YZJFyzqKkCM4nUPs-BA9GbrztlN9KSuTOqIxG5dFoDLzYdx7rDpojPimM9Zf7ugpatcYrp204YDyL1sWuTTlhP6HuTdAWnIYDtaBCsKuFiAPs8MoOame16kc3xOir_49GWuxp28L2H9vJy5v1H7smU9aGAX4dssp_k3mRFpn8_OFCsi-ry_Vdupbvj0tBfNofFrzcT9dYH3-VbHr7N62_AX2p0Rw</recordid><startdate>199212</startdate><enddate>199212</enddate><creator>Bikhazi, Anwar B.</creator><creator>Medlej‐Hashim, Myrna</creator><creator>El‐Kasti, Muna</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>AMER PHARMACEUTICAL ASSN</general><general>Wiley</general><general>American Pharmaceutical Association</general><scope>BSCLL</scope><scope>BLEPL</scope><scope>DTL</scope><scope>EZCTM</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199212</creationdate><title>Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys</title><author>Bikhazi, Anwar B. ; Medlej‐Hashim, Myrna ; El‐Kasti, Muna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4560-3e0f6478184cb1b4ef86a44c4489a25ff87222a6c5cccfb941ad22edec09f96f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Chemistry</topic><topic>Chemistry, Medicinal</topic><topic>Chemistry, Multidisciplinary</topic><topic>Ethacrynic Acid - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kidney - drug effects</topic><topic>Kidney - metabolism</topic><topic>Kinetics</topic><topic>Life Sciences &amp; Biomedicine</topic><topic>Ouabain - pharmacology</topic><topic>Perfusion</topic><topic>Pharmacology &amp; Pharmacy</topic><topic>Physical Sciences</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Science &amp; Technology</topic><topic>Sodium - physiology</topic><topic>Verapamil - pharmacology</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bikhazi, Anwar B.</creatorcontrib><creatorcontrib>Medlej‐Hashim, Myrna</creatorcontrib><creatorcontrib>El‐Kasti, Muna</creatorcontrib><collection>Istex</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 1992</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bikhazi, Anwar B.</au><au>Medlej‐Hashim, Myrna</au><au>El‐Kasti, Muna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><stitle>J PHARM SCI</stitle><addtitle>J. Pharm. Sci</addtitle><date>1992-12</date><risdate>1992</risdate><volume>81</volume><issue>12</issue><spage>1178</spage><epage>1180</epage><pages>1178-1180</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><coden>JPMSAE</coden><abstract>A Sprague‐Dawley rat kidney perfusion technique was used in situ to study the effect of verapamil, ouabain, and ethacrynic acid on renal calcium retention. The technique involves perfusion of the kidneys via the abdominal aorta and then through the left and right renal arteries and dorsal aorta. Verapamil (1mM) in Krebs‐improved Ringer solution increased calcium retention in the kidneys by ~ 117% compared with controls. With Na‐free Krebs‐improved Ringer solution, calcium retention increased by only 92%. However, in Krebs‐improved Ringer solutions containing 59 and 122 mequivalents of Na, calcium retention in the kidney increased by 46 and 43%, respectively, compared with controls. With Krebs‐improved Ringer solution containing 15mM ouabain, calcium retention in the kidney decreased by 29.5%, whereas with 15mM ouabain plus 1mM ethacrynic acid in the perfusate, the effect on calcium retention in the kidney was insignificant compared with controls. These results suggest that two sodium‐dependent calcium‐transporting systems exist at the peritubular side of the kidney tubules: (1) a Na+–Ca+2 countertransport system sensitive to verapamil and (2) a Na+–Ca+2 cotransport system sensitive to the intracellular concentration of sodium.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>1491335</pmid><doi>10.1002/jps.2600811210</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-3549
ispartof Journal of pharmaceutical sciences, 1992-12, Vol.81 (12), p.1178-1180
issn 0022-3549
1520-6017
language eng
recordid cdi_proquest_miscellaneous_73442081
source Wiley Online Library Journals
subjects Animals
Biological and medical sciences
Calcium - metabolism
Calcium-Binding Proteins - metabolism
Chemistry
Chemistry, Medicinal
Chemistry, Multidisciplinary
Ethacrynic Acid - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Kidney - drug effects
Kidney - metabolism
Kinetics
Life Sciences & Biomedicine
Ouabain - pharmacology
Perfusion
Pharmacology & Pharmacy
Physical Sciences
Rats
Rats, Sprague-Dawley
Science & Technology
Sodium - physiology
Verapamil - pharmacology
Vertebrates: urinary system
title Effects of Verapamil, Ouabain, and Ethacrynic Acid on Calcium Retention in Perfused Rat Kidneys
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T17%3A15%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Verapamil,%20Ouabain,%20and%20Ethacrynic%20Acid%20on%20Calcium%20Retention%20in%20Perfused%20Rat%20Kidneys&rft.jtitle=Journal%20of%20pharmaceutical%20sciences&rft.au=Bikhazi,%20Anwar%20B.&rft.date=1992-12&rft.volume=81&rft.issue=12&rft.spage=1178&rft.epage=1180&rft.pages=1178-1180&rft.issn=0022-3549&rft.eissn=1520-6017&rft.coden=JPMSAE&rft_id=info:doi/10.1002/jps.2600811210&rft_dat=%3Cproquest_cross%3E73442081%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4560-3e0f6478184cb1b4ef86a44c4489a25ff87222a6c5cccfb941ad22edec09f96f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=73442081&rft_id=info:pmid/1491335&rfr_iscdi=true