RP-HPLC study of the degradation of diclofenac and piroxicam in the presence of hydroxyl radicals

The effect of hydroxyl radical attack on two non-steroidal anti-inflammatory drugs (NSAIDs) was studied in vitro. Diclofenac and piroxicam were analysed by RP-HPLC after reaction with OH free radicals to detect newly formed oxidation and/or degradation products. OH free radicals were obtained by mea...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2003-04, Vol.32 (1), p.151-158
Main Authors: Gaudiano, M.C, Valvo, L, Bertocchi, P, Manna, L
Format: Article
Language:English
Subjects:
Analysis
Anti-Inflammatory Agents, Non-Steroidal - analysis
Anti-Inflammatory Agents, Non-Steroidal - chemistry
Biological and medical sciences
Chromatography, High Pressure Liquid
Cyclooxygenase Inhibitors - analysis
Cyclooxygenase Inhibitors - chemistry
Degradation
Diclofenac
Diclofenac - analysis
Diclofenac - chemistry
Drug Stability
Drugs
General pharmacology
Hydroxyl Radical - chemistry
Hydroxyl radicals
Medical sciences
Oxidation
Oxidation-Reduction
Pharmacology. Drug treatments
Piroxicam
Piroxicam - analysis
Piroxicam - chemistry
RP-HPLC
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Summary:The effect of hydroxyl radical attack on two non-steroidal anti-inflammatory drugs (NSAIDs) was studied in vitro. Diclofenac and piroxicam were analysed by RP-HPLC after reaction with OH free radicals to detect newly formed oxidation and/or degradation products. OH free radicals were obtained by means of ferrous sulphate and ascorbic acid mixtures. During the reaction the mixtures were exposed to irradiation by a tungsten lamp to obtain an increased and more reproducible formation of hydroxyl radicals. The chromatographic profiles showed the formation of several new peaks for both diclofenac and piroxicam due to the presence of a number of degradation/oxidation products formed in the presence of OH radicals.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(03)00058-X