New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing down syndrome chromosomal region

To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. Th...

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Bibliographic Details
Published in:Human genetics 1992-12, Vol.90 (4), p.427-434
Main Authors: YASPO, M.-L, CRETE, N, CHETTOUH, Z, BLOUIN, J.-L, RAHMANI, Z, STEHELIN, D, SINET, P.-M, CRAU-GOLDBERG, N, DELABAR, J.-M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 21
Classical genetics, quantitative genetics, hybrids
Cricetinae
DNA
Down Syndrome - genetics
Down's syndrome
Electrophoresis, Gel, Pulsed-Field
ETS2 gene
Fundamental and applied biological sciences. Psychology
gel electrophoresis
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Hybrid Cells
man
markers
Mice
Oncogenes
Polymorphism, Genetic
Retroviridae Proteins, Oncogenic - genetics
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Summary:To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. This fragment, which contains the oncogene ETS2, was used to construct a partial genomic library. Among the 14 unique sequences that were isolated, 3 were polymorphic markers and contained sequences that are conserved in mammals. Five of these markers mapped on the ETS2-containing NruI fragment and allowed us to define an 800-kb high-resolution PFGE map.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00220472