Determination of dietary fibre as non-starch polysaccharides by gas-liquid chromatography

An improved method is described for the measurement of total, soluble and insoluble dietary fibre as non-starch polysaccharides (NSP). An established procedure is modified to allow more rapid removal of starch and hydrolysis of NSP. In its present form the procedure is simpler and more robust than t...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 1992, Vol.117 (11), p.1707-1714
Main Authors: ENGLYST, H. N, QUIGLEY, M. E, HUDSON, G. J, CUMMINGS, J. H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, Gas - methods
Chromatography, High Pressure Liquid
Dietary Fiber - analysis
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Hydrolysis
Methods of analysis, processing and quality control, regulation, standards
Polysaccharides - analysis
Sulfuric Acids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An improved method is described for the measurement of total, soluble and insoluble dietary fibre as non-starch polysaccharides (NSP). An established procedure is modified to allow more rapid removal of starch and hydrolysis of NSP. In its present form the procedure is simpler and more robust than those previously published. In the modified method starch is removed enzymically within 50 min and NSP is precipitated with ethanol and then hydrolysed by treatment with sulfuric acid for 2 h. The constituent sugars can in turn be measured by gas-liquid chromatography, high-performance liquid chromatography or more rapidly by colorimetry. The improved procedure described here for the removal of starch and hydrolysis of NSP applies to all three techniques, but only the method for measurement of sugars by gas-liquid chromatography is described here in full.
ISSN:0003-2654
1364-5528
DOI:10.1039/AN9921701707