Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae)

Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design po...

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Bibliographic Details
Published in:Journal of economic entomology 2003-06, Vol.96 (3), p.669-672
Main Author: Roehrdanz, Richard L.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Coleoptera - classification
Coleoptera - genetics
corn rootworm
cytochrome-c oxidase
Diabrotica
Diabrotica barberi
Diabrotica virgifera virgifera
DNA primers
FIELD AND FORAGE CROPS
Fundamental and applied biological sciences. Psychology
Generalities
insect pests
insect taxonomy
Insecta
Invertebrates
Larva - classification
Larva - genetics
larvae
mitochondrial DNA
molecular equence data
molecular systematics
nucleotide sequences
pest identification
Phytopathology. Animal pests. Plant and forest protection
polymerase chain reaction
Polymerase Chain Reaction - methods
Protozoa. Invertebrates
species diagnostics
Species Specificity
sympatric species
Systematics. Geographical distribution
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Summary:Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species.
ISSN:0022-0493
1938-291X
DOI:10.1603/0022-0493-96.3.669