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Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae)
Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design po...
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| Published in: | Journal of economic entomology 2003-06, Vol.96 (3), p.669-672 |
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| Format: | Article |
| Language: | English |
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| container_end_page | 672 |
| container_issue | 3 |
| container_start_page | 669 |
| container_title | Journal of economic entomology |
| container_volume | 96 |
| creator | Roehrdanz, Richard L. |
| description | Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species. |
| doi_str_mv | 10.1603/0022-0493-96.3.669 |
| format | article |
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Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species.</description><identifier>ISSN: 0022-0493</identifier><identifier>EISSN: 1938-291X</identifier><identifier>DOI: 10.1603/0022-0493-96.3.669</identifier><identifier>PMID: 12852603</identifier><identifier>CODEN: JEENAI</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Animals ; Biological and medical sciences ; Coleoptera - classification ; Coleoptera - genetics ; corn rootworm ; cytochrome-c oxidase ; Diabrotica ; Diabrotica barberi ; Diabrotica virgifera virgifera ; DNA primers ; FIELD AND FORAGE CROPS ; Fundamental and applied biological sciences. Psychology ; Generalities ; insect pests ; insect taxonomy ; Insecta ; Invertebrates ; Larva - classification ; Larva - genetics ; larvae ; mitochondrial DNA ; molecular equence data ; molecular systematics ; nucleotide sequences ; pest identification ; Phytopathology. Animal pests. Plant and forest protection ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Protozoa. Invertebrates ; species diagnostics ; Species Specificity ; sympatric species ; Systematics. 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Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Coleoptera - classification</subject><subject>Coleoptera - genetics</subject><subject>corn rootworm</subject><subject>cytochrome-c oxidase</subject><subject>Diabrotica</subject><subject>Diabrotica barberi</subject><subject>Diabrotica virgifera virgifera</subject><subject>DNA primers</subject><subject>FIELD AND FORAGE CROPS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities</subject><subject>insect pests</subject><subject>insect taxonomy</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Larva - classification</subject><subject>Larva - genetics</subject><subject>larvae</subject><subject>mitochondrial DNA</subject><subject>molecular equence data</subject><subject>molecular systematics</subject><subject>nucleotide sequences</subject><subject>pest identification</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protozoa. Invertebrates</subject><subject>species diagnostics</subject><subject>Species Specificity</subject><subject>sympatric species</subject><subject>Systematics. 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Psychology</topic><topic>Generalities</topic><topic>insect pests</topic><topic>insect taxonomy</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Larva - classification</topic><topic>Larva - genetics</topic><topic>larvae</topic><topic>mitochondrial DNA</topic><topic>molecular equence data</topic><topic>molecular systematics</topic><topic>nucleotide sequences</topic><topic>pest identification</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protozoa. Invertebrates</topic><topic>species diagnostics</topic><topic>Species Specificity</topic><topic>sympatric species</topic><topic>Systematics. Geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roehrdanz, Richard L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of economic entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roehrdanz, Richard L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae)</atitle><jtitle>Journal of economic entomology</jtitle><addtitle>J Econ Entomol</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>96</volume><issue>3</issue><spage>669</spage><epage>672</epage><pages>669-672</pages><issn>0022-0493</issn><eissn>1938-291X</eissn><coden>JEENAI</coden><abstract>Western corn rootworm, Diabrotica virgifera virgifera LeConte, and northern corn rootworm, D. barberi Smith and Lawrence, are sympatric species and serious pests of corn cultivation in North America. Comparison of nucleotide sequence of mitochondrial cytochrome oxidase I and II was used to design polymerase chain reaction (PCR) primers that discriminate immature stages of the two species based on differences in amplicon size. Multiplex PCR can be used to give a positive test for each species in a single amplification reaction. This provides a method to identify field caught larvae and facilitates investigations of larval interaction and competition between the species.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>12852603</pmid><doi>10.1603/0022-0493-96.3.669</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-0493 |
| ispartof | Journal of economic entomology, 2003-06, Vol.96 (3), p.669-672 |
| issn | 0022-0493 1938-291X |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Coleoptera - classification Coleoptera - genetics corn rootworm cytochrome-c oxidase Diabrotica Diabrotica barberi Diabrotica virgifera virgifera DNA primers FIELD AND FORAGE CROPS Fundamental and applied biological sciences. Psychology Generalities insect pests insect taxonomy Insecta Invertebrates Larva - classification Larva - genetics larvae mitochondrial DNA molecular equence data molecular systematics nucleotide sequences pest identification Phytopathology. Animal pests. Plant and forest protection polymerase chain reaction Polymerase Chain Reaction - methods Protozoa. Invertebrates species diagnostics Species Specificity sympatric species Systematics. Geographical distribution |
| title | Multiplex Polymerase Chain Reaction Method for Differentiating Western and Northern Corn Rootworm Larvae (Coleoptera: Chrysomelidae) |
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