Applications of environmental scanning electron microscopy (ESEM) in biomaterials field

Various tasks were undertaken in our laboratory where environmental scanning electron microscopy (ESEM) has been of particular interest within the biomaterials field. The possibility of observing wet samples, as well as the fact that sample preparation is minimal, has improved shorter time scales an...

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Bibliographic Details
Published in:Microscopy research and technique 2003-08, Vol.61 (5), p.469-480
Main Authors: Manero, J.M., Gil, F.J., Padrós, E., Planell, J.A.
Format: Article
Language:English
Subjects:
Abdominal Wall - pathology
Animals
bioactive surfaces
Biocompatible Materials - analysis
Bone Resorption - pathology
cell cultures
Durapatite - analysis
Environment
ESEM
Humans
Microscopy, Electron, Scanning - methods
Osteoclasts - ultrastructure
phosphate glasses
Polypropylenes
shape memory alloys
Surgical Mesh
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Summary:Various tasks were undertaken in our laboratory where environmental scanning electron microscopy (ESEM) has been of particular interest within the biomaterials field. The possibility of observing wet samples, as well as the fact that sample preparation is minimal, has improved shorter time scales and lower costs in microscopy. Minimal preparation has also reduced the possibility of introducing artifacts. Examples like cell cultures used for pit resorption assays, calcium phosphate deposition processes, and dissolution of phosphate glasses used as biomaterials are presented. Finally, a servohydraulic testing machine designed for mechanical testing in situ in ESEM has allowed the study of shape memory alloys for orthodontic applications or the behavior of different adhesives used in odontology. Microsc. Res. Tech. 61:469–480, 2003. © 2003 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.10358