Regional mRNA changes in brain stem motor neurons from patients with amyotrophic lateral sclerosis

An antisense oligonucleotide (54 mer) from the mRNA to the midsize neurofilament protein (NFM) was labeled with 35S on the 3' end and purified by polyacrylamide gel electrophoresis (PAGE). In situ hybridization was performed on sections from medulla oblongata of patients with amyotrophic latera...

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Bibliographic Details
Published in:Molecular and chemical neuropathology 1992-12, Vol.17 (3), p.249-257
Main Authors: Hartmann, H A, Sun, D Y
Format: Article
Language:English
Subjects:
Aged
Amyotrophic Lateral Sclerosis - metabolism
Amyotrophic Lateral Sclerosis - pathology
Autoradiography
Base Sequence
Brain Stem - metabolism
Brain Stem - pathology
Electrophoresis, Polyacrylamide Gel
Female
Humans
Hypoglossal Nerve - pathology
In Situ Hybridization
Male
Middle Aged
Molecular Sequence Data
Motor Neurons - metabolism
Neurofilament Proteins - biosynthesis
Oligonucleotides, Antisense
RNA, Messenger - metabolism
Transcription, Genetic
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Summary:An antisense oligonucleotide (54 mer) from the mRNA to the midsize neurofilament protein (NFM) was labeled with 35S on the 3' end and purified by polyacrylamide gel electrophoresis (PAGE). In situ hybridization was performed on sections from medulla oblongata of patients with amyotrophic lateral sclerosis (ALS). The slides were dipped in photographic emulsion, developed, and stained. Neurons from both nucleus hypoglossus and nucleus ambiguous showed a marked reduction of silver grains when compared to normal. This indicates a reduction of mRNA, which may precede the reduction of ribosomal RNA and the changes in neurofilament proteins that have been described by several investigators in ALS. It does not settle the question of whether the reduction of mRNA is owing to reduced transcription or increased decay of mRNA.
ISSN:1044-7393
DOI:10.1007/BF03160014