Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion

• Published in: Immunology letters 2003-07, Vol.88 (1), p.27-30
• Main Authors: Yang, Hengwen, Lan, Canhui, Xiao, Yi, Chen, Ying-Hua
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Animals; Antibodies, Monoclonal; CD14; Cell fusion; Cells, Cultured; Chemotaxis; Chemotaxis, Leukocyte; CHO Cells; Cricetinae; Cricetulus; CXCR4; HIV coreceptor; HIV-1 - physiology; Humans; Lipopolysaccharide Receptors - immunology; Lipopolysaccharide Receptors - physiology; Membrane Fusion; Mice; Monocytes - physiology; Receptors, CXCR4 - genetics; Receptors, CXCR4 - immunology; Receptors, CXCR4 - physiology; Receptors, HIV - immunology; Receptors, HIV - physiology; Transfection; U937 Cells
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/S0165-2478(03)00048-8

The expression of HIV-1 coreceptors (CXCR4 and CCR5) on monocyte surface can be regulated by the ligand of CD14 (LPS), which stimulate the susceptibility of the cells to HIV-1. To investigate whether it exists potential association between CD14 and HIV-1 coreceptor CXCR4, we tested the impact of CD14-specific monoclonal antibodies (mAbs) upon CXCR4-dependent responses, such as SDF-induced chemotaxis and HIV Env-mediated membrane fusion. The anti-CD14 mAb TUK4 like CXCR4-specific mAb 12G5 could block SDF-induced chemotaxis of U937 cells in a dose-dependent manner, while another CD14-specific mAb UCHM-1 did not show any activity. More interestingly, syncytium assay indicated that only the CD14-specific mAb TUK4 inhibited HIV Env-mediated CXCR4-dependent cell fusion between U937 cells and HIV-1 HXB2 Env transfected CHO cells distinctly, consistent with its activity against CXCR4-dependent chemotaxis. These results provided experimental evidence for existence of close association between CD14 and HIV coreceptor CXCR4 on human monocytic cells.