Determination by HPLC of chlortetracycline in pig faeces

An HPLC assay used to determine chlortetracycline (CTC) in pig faeces is reported. Prodigy ODS3 (4.6 × 150 mm) was used for the stationary phase, whereas the mobile phase comprised oxalic acid, sodium oxalate and sodium decane sulfonate (66%)—each of 4 mM, and 34% acetonitrile. The mobile phase was...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2003-07, Vol.52 (1), p.135-137
Main Authors: Sunderland, Julie, Lovering, Andrew M., Tobin, Caroline M., MacGowan, Alasdair P., Roe, John M., Delsol, Anne A.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - analysis
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
chlortetracycline
Chlortetracycline - analysis
Chromatography, High Pressure Liquid
Feces - chemistry
HPLC
Medical sciences
Pharmacology. Drug treatments
pig faeces
Reproducibility of Results
Swine
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Summary:An HPLC assay used to determine chlortetracycline (CTC) in pig faeces is reported. Prodigy ODS3 (4.6 × 150 mm) was used for the stationary phase, whereas the mobile phase comprised oxalic acid, sodium oxalate and sodium decane sulfonate (66%)—each of 4 mM, and 34% acetonitrile. The mobile phase was pumped at a flow rate of 1 mL/min. Detection of CTC was by ultraviolet absorbance at 370 nm, and a 20 µL injection volume was used. Recovery from faeces was >90%, and coefficients of variability between runs were
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/dkg279