Na +-dependent fructose transport via rNaGLT1 in rat kidney

We found a system of Na +-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as α-methyl- D-glucop...

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Bibliographic Details
Published in:FEBS letters 2003-07, Vol.546 (2), p.276-280
Main Authors: Horiba, Naoshi, Masuda, Satohiro, Ohnishi, Chiharu, Takeuchi, Daisuke, Okuda, Masahiro, Inui, Ken-ichi
Format: Article
Language:English
Subjects:
2-DG, 2-deoxy-D-glucose
Animals
Biological Transport
Brush-border membrane vesicle
Cell Line
Fructose
Fructose - metabolism
Humans
Kidney
Kidney - metabolism
Microvilli - metabolism
Monosaccharide Transport Proteins - metabolism
Na +-dependent
Rat Na +-dependent glucose transporter 1
Rats
Recombinant Proteins - metabolism
rNaGLT1, rat Na+-dependent glucose transporter 1
Sodium - metabolism
αMeGlc, α-methyl-D-glucopyranoside
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Summary:We found a system of Na +-dependent uptake of fructose by rat renal brush-border membrane vesicles. It consisted of two saturable components, and was thought to involve at least two transporters. rNaGLT1, a novel glucose transporter in rat kidney, showed fructose uptake as well as α-methyl- D-glucopyranoside uptake by transfected HEK293 cells. The features of the lower affinity type of fructose transporter in the brush-border membranes, such as affinity and substrate recognition, were very comparable with those of rNaGLT1-transfected HEK293 cells. These results indicated that rNaGLT1 is a primary fructose transporter in rat renal brush-border membranes.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00600-8