Observation and analysis of single DNA nano-kinetics by pin-fiber video scope

The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measure...

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Bibliographic Details
Published in:Analyst (London) 2003-06, Vol.128 (6), p.676-680
Main Authors: Hirakawa, Yasuyuki, Suzuto, Masashi, Ohnishi, Hitoshi, Shingaki, Tomoteru, Eyring, Edward M, Tokunaga, Makio, Masujima, Tsutomu
Format: Article
Language:English
Subjects:
DNA - metabolism
Fiber Optic Technology
Humans
Microscopy, Video
Nanotechnology - methods
Optical Fibers
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Summary:The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule.
ISSN:0003-2654
1364-5528
DOI:10.1039/b212830f