Axial resolution limit of a fiber-optic fluorescence probe

We examine the limit of spatial resolution achievable when a sine optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wave...

Full description

Saved in:
Bibliographic Details
Published in:Applied optics (2004) 2003-07, Vol.42 (19), p.3780-3784
Main Authors: Balaji, J, Garai, Kanchan, Chakrabarti, Subhasis, Maiti, Sudipta
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c287t-ad2e5f87083a17adee3f7c38ace2301d2e2bccc5d59e5bdfa81d145a3e3a96a73
cites
container_end_page 3784
container_issue 19
container_start_page 3780
container_title Applied optics (2004)
container_volume 42
creator Balaji, J
Garai, Kanchan
Chakrabarti, Subhasis
Maiti, Sudipta
description We examine the limit of spatial resolution achievable when a sine optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength lambda, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is approximately 10 microm. We experimentally measured the axial resolution for a 500-microM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 microm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.
doi_str_mv 10.1364/AO.42.003780
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73469347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73469347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c287t-ad2e5f87083a17adee3f7c38ace2301d2e2bccc5d59e5bdfa81d145a3e3a96a73</originalsourceid><addsrcrecordid>eNo1j0tLxDAURrNQnHF051qyctfavJrU3TA4KgzMRsFduU1vIJI2tWlB_70Vx9UH5zvcByE3rMiZKOX99phLnheF0KY4I2umVJUxbt5X5DKlj4UrWekLslpYaQxTa_Kw_fIQ6IgphnnysafBd36i0VGgzjc4ZnGYvKUuzHGxLPYW6TDGBq_IuYOQ8PqUG_K2f3zdPWeH49PLbnvILDd6yqDlqJzRhRHANLSIwmkrDFjkomBLyxtrrWpVhappHRjWMqlAoICqBC025O5v7rL1c8Y01Z1f7ggBeoxzqrWQZSXkr3h7Euemw7YeRt_B-F3_fyt-ALfXVPo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73469347</pqid></control><display><type>article</type><title>Axial resolution limit of a fiber-optic fluorescence probe</title><source>Optica Publishing Group Journals</source><creator>Balaji, J ; Garai, Kanchan ; Chakrabarti, Subhasis ; Maiti, Sudipta</creator><creatorcontrib>Balaji, J ; Garai, Kanchan ; Chakrabarti, Subhasis ; Maiti, Sudipta</creatorcontrib><description>We examine the limit of spatial resolution achievable when a sine optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength lambda, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is approximately 10 microm. We experimentally measured the axial resolution for a 500-microM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 microm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.</description><identifier>ISSN: 1559-128X</identifier><identifier>DOI: 10.1364/AO.42.003780</identifier><identifier>PMID: 12868815</identifier><language>eng</language><publisher>United States</publisher><ispartof>Applied optics (2004), 2003-07, Vol.42 (19), p.3780-3784</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c287t-ad2e5f87083a17adee3f7c38ace2301d2e2bccc5d59e5bdfa81d145a3e3a96a73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12868815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balaji, J</creatorcontrib><creatorcontrib>Garai, Kanchan</creatorcontrib><creatorcontrib>Chakrabarti, Subhasis</creatorcontrib><creatorcontrib>Maiti, Sudipta</creatorcontrib><title>Axial resolution limit of a fiber-optic fluorescence probe</title><title>Applied optics (2004)</title><addtitle>Appl Opt</addtitle><description>We examine the limit of spatial resolution achievable when a sine optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength lambda, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is approximately 10 microm. We experimentally measured the axial resolution for a 500-microM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 microm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.</description><issn>1559-128X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNo1j0tLxDAURrNQnHF051qyctfavJrU3TA4KgzMRsFduU1vIJI2tWlB_70Vx9UH5zvcByE3rMiZKOX99phLnheF0KY4I2umVJUxbt5X5DKlj4UrWekLslpYaQxTa_Kw_fIQ6IgphnnysafBd36i0VGgzjc4ZnGYvKUuzHGxLPYW6TDGBq_IuYOQ8PqUG_K2f3zdPWeH49PLbnvILDd6yqDlqJzRhRHANLSIwmkrDFjkomBLyxtrrWpVhappHRjWMqlAoICqBC025O5v7rL1c8Y01Z1f7ggBeoxzqrWQZSXkr3h7Euemw7YeRt_B-F3_fyt-ALfXVPo</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Balaji, J</creator><creator>Garai, Kanchan</creator><creator>Chakrabarti, Subhasis</creator><creator>Maiti, Sudipta</creator><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>Axial resolution limit of a fiber-optic fluorescence probe</title><author>Balaji, J ; Garai, Kanchan ; Chakrabarti, Subhasis ; Maiti, Sudipta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c287t-ad2e5f87083a17adee3f7c38ace2301d2e2bccc5d59e5bdfa81d145a3e3a96a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balaji, J</creatorcontrib><creatorcontrib>Garai, Kanchan</creatorcontrib><creatorcontrib>Chakrabarti, Subhasis</creatorcontrib><creatorcontrib>Maiti, Sudipta</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Applied optics (2004)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balaji, J</au><au>Garai, Kanchan</au><au>Chakrabarti, Subhasis</au><au>Maiti, Sudipta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Axial resolution limit of a fiber-optic fluorescence probe</atitle><jtitle>Applied optics (2004)</jtitle><addtitle>Appl Opt</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>42</volume><issue>19</issue><spage>3780</spage><epage>3784</epage><pages>3780-3784</pages><issn>1559-128X</issn><abstract>We examine the limit of spatial resolution achievable when a sine optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength lambda, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is approximately 10 microm. We experimentally measured the axial resolution for a 500-microM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 microm, and a N.A. of 0.16 and found good qualitative agreement with the calculation.</abstract><cop>United States</cop><pmid>12868815</pmid><doi>10.1364/AO.42.003780</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1559-128X
ispartof Applied optics (2004), 2003-07, Vol.42 (19), p.3780-3784
issn 1559-128X
language eng
recordid cdi_proquest_miscellaneous_73469347
source Optica Publishing Group Journals
title Axial resolution limit of a fiber-optic fluorescence probe
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T12%3A22%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Axial%20resolution%20limit%20of%20a%20fiber-optic%20fluorescence%20probe&rft.jtitle=Applied%20optics%20(2004)&rft.au=Balaji,%20J&rft.date=2003-07-01&rft.volume=42&rft.issue=19&rft.spage=3780&rft.epage=3784&rft.pages=3780-3784&rft.issn=1559-128X&rft_id=info:doi/10.1364/AO.42.003780&rft_dat=%3Cproquest_pubme%3E73469347%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c287t-ad2e5f87083a17adee3f7c38ace2301d2e2bccc5d59e5bdfa81d145a3e3a96a73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=73469347&rft_id=info:pmid/12868815&rfr_iscdi=true