Genotyping of Chlamydia trachomatis strains from cultured isolates and nucleic acid amplification test-positive specimens

Urogenital strains of Chlamydia trachomatis are divided into several serogroups (D – K). Since these serovars are represented with differing prevalence in the population a serotyping of strains is necessary, when characterising the epidemiological situation. The aim of this study was the genotyping...

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Published in:International journal of medical microbiology 2003-06, Vol.293 (2-3), p.225-228
Main Authors: Oehme, Albrecht, Gaschler, Gisela, Straube, Eberhard
Format: Article
Language:English
Subjects:
Ascites - microbiology
Biological and medical sciences
Cervix Uteri - microbiology
Chlamydia Infections - epidemiology
Chlamydia Infections - microbiology
Chlamydia trachomatis
Chlamydia trachomatis - classification
Chlamydia trachomatis - genetics
Chlamydia trachomatis - isolation & purification
Eye - microbiology
Female
Fundamental and applied biological sciences. Psychology
Genotype
genotyping
Germany - epidemiology
Hospitals, Private
Hospitals, University
Humans
Microbiology
Molecular Epidemiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Porins - genetics
Urethra - microbiology
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Gaschler, Gisela
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description Urogenital strains of Chlamydia trachomatis are divided into several serogroups (D – K). Since these serovars are represented with differing prevalence in the population a serotyping of strains is necessary, when characterising the epidemiological situation. The aim of this study was the genotyping of C. trachomatis strains, the comparison of the results with those of serotyping, and the genotyping of positive specimens using commercial nucleic acid amplification tests (NAAT). The Chlamydia trachomatis major outer membrane protein gene (omp1) from 55 isolated strains and 36 NAAT-positive specimens was amplified by polymerase chain reaction (PCR). The restriction fragment length polymorphism (RFLP) patterns of these amplicons were compared with those of reference strains. The genotypes E and F were found to be most prevalent. The results are discussed considering other studies, genovariants and epidemiology.
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Since these serovars are represented with differing prevalence in the population a serotyping of strains is necessary, when characterising the epidemiological situation. The aim of this study was the genotyping of C. trachomatis strains, the comparison of the results with those of serotyping, and the genotyping of positive specimens using commercial nucleic acid amplification tests (NAAT). The Chlamydia trachomatis major outer membrane protein gene (omp1) from 55 isolated strains and 36 NAAT-positive specimens was amplified by polymerase chain reaction (PCR). The restriction fragment length polymorphism (RFLP) patterns of these amplicons were compared with those of reference strains. The genotypes E and F were found to be most prevalent. 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subjects Ascites - microbiology
Biological and medical sciences
Cervix Uteri - microbiology
Chlamydia Infections - epidemiology
Chlamydia Infections - microbiology
Chlamydia trachomatis
Chlamydia trachomatis - classification
Chlamydia trachomatis - genetics
Chlamydia trachomatis - isolation & purification
Eye - microbiology
Female
Fundamental and applied biological sciences. Psychology
Genotype
genotyping
Germany - epidemiology
Hospitals, Private
Hospitals, University
Humans
Microbiology
Molecular Epidemiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Porins - genetics
Urethra - microbiology
title Genotyping of Chlamydia trachomatis strains from cultured isolates and nucleic acid amplification test-positive specimens
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