'Candidatus Rickettsia tarasevichiae' in Ixodes persulcatus Ticks Collected in Russia

: We collected 209 Ixodes persulcatus ticks in various regions of Russia, including the southern Urals and western and eastern Siberia. Using PCR amplification and sequencing of the citrate synthase‐encoding gene (gltA), we detected a new rickettsial genotype, which we named ‘Candidatus Rickettsia t...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2003-06, Vol.990 (1), p.162-172
Main Authors: SHPYNOV, STANISLAV, FOURNIER, PIERRE-EDOUARD, RUDAKOV, NIKOLAY, RAOULT, DIDIER
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Candidatus Rickettsia tarasevichiae
Citrate (si)-Synthase - genetics
DNA Primers
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Genotype
Geography
Ixodes - microbiology
Ixodes persulcatus
Ixodidae
Phylogeny
Polymerase Chain Reaction
Rickettsia
Rickettsia - classification
Rickettsia - genetics
Rickettsia - isolation & purification
RNA, Ribosomal, 16S - genetics
Russia
Siberia
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Summary:: We collected 209 Ixodes persulcatus ticks in various regions of Russia, including the southern Urals and western and eastern Siberia. Using PCR amplification and sequencing of the citrate synthase‐encoding gene (gltA), we detected a new rickettsial genotype, which we named ‘Candidatus Rickettsia tarasevichiae.’ This bacterium was found in 9.27%, 10.0%, and 20.5% of the ticks collected in western Siberia, eastern Siberia, and the southern Urals, respectively. ‘Candidatus Rickettsia tarasevichiae’ exhibited a 98% and 96% nucleotide sequence homology, with the 16S rDNA and gltA sequence, respectively, of R. canadensis, a rickettsia previously only found in Haemaphysalis leporispalustris ticks in North America. The phylogenetic analysis of ‘Candidatus Rickettsia tarasevichiae’ and other Rickettsia species allowed the creation of a new cluster with high bootstrap values within the Rickettsia genus involving this rickettsia, R. canadensis, and three uncultured rickettsiae from plant insects.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.2003.tb07358.x