Structural analysis of the carbohydrate chain of glycopeptides isolated from Robinia pseudoacacia seed lectins

Robinia pseudoacacia seeds contain lectins which are closely related. Pronase digestion of the dimeric and tetrameric lectins, RPA1 and RPA3, gave glycopeptides. The structure of the oligosaccharide was determined by 1H NMR spectroscopy and carbohydrate determination as α- d-Man p-(1 → 3)-[(β- d-Xyl...

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Bibliographic Details
Published in:Carbohydrate research 1992-12, Vol.236, p.181-193
Main Authors: Wantyghem, Josiane, Platzer, Nicole, Giner, Mireille, Derappe, Christian, Goussault, Yves
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Sequence
Carbohydrates - chemistry
Fundamental and applied biological sciences. Psychology
Glycopeptides - chemistry
Glycopeptides - isolation & purification
Glycoproteins
Lectins - analysis
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Plant Lectins
Proteins
Protons
Seeds - chemistry
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Summary:Robinia pseudoacacia seeds contain lectins which are closely related. Pronase digestion of the dimeric and tetrameric lectins, RPA1 and RPA3, gave glycopeptides. The structure of the oligosaccharide was determined by 1H NMR spectroscopy and carbohydrate determination as α- d-Man p-(1 → 3)-[(β- d-Xylp-(1 → 2)]-[α- d-Man p-(1 → 6)]-β- d-Man p-(1 → 4)-β- d-Glc pNAc-(1 → 4)-[α- l-Fuc p-(1 → 3)]-β- d-Glc pNAc-(1 → 4)-Asn. It appears that the 34-kDa constitutive polypeptide of RPA1 contains 4-5 carbohydrate chains whereas the 30.5-kDa and 29-kDa subunits of RPA3 contain two and one oligosaccharide chains, respectively.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(92)85015-R