Increased plasma DNA integrity in cancer patients

Tumor-released DNA in blood represents a promising biomarker for cancer detection. It has been postulated that tumor necrosis causes release of DNA of varying sizes, which contrasts apoptosis in normal tissue that releases smaller and more uniform DNA fragments. To test the hypothesis that increased...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2003-07, Vol.63 (14), p.3966-3968
Main Authors: WANG, Brant G, HUANG, Han-Yao, CHEN, Yu-Chi, BRISTOW, Robert E, KASSAUEI, Keyanunoosh, CHENG, Chih-Chien, RODEN, Richard, SOKOLL, Lori J, CHAN, Daniel W, SHIH, Ie-Ming
Format: Article
Language:English
Subjects:
Biological and medical sciences
Case-Control Studies
DNA, Neoplasm - blood
DNA, Neoplasm - genetics
Female
Genital Neoplasms, Female - genetics
Humans
Medical sciences
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Reverse Transcriptase Polymerase Chain Reaction
ROC Curve
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tumor-released DNA in blood represents a promising biomarker for cancer detection. It has been postulated that tumor necrosis causes release of DNA of varying sizes, which contrasts apoptosis in normal tissue that releases smaller and more uniform DNA fragments. To test the hypothesis that increased DNA integrity, i.e., a longer DNA strand, is a tumor-associated marker in plasma, we determined the genomic DNA integrity index in plasma DNA using real-time PCR assays. A DNA integrity index and DNA concentration in plasma were determined in 61 patients with gynecological and breast cancers and 65 female patients without neoplastic diseases. We found that the area under the receiver-operating characteristic curve for DNA integrity index was 0.911 for cancer versus nonneoplastic patients. Given 100% specificity, the highest sensitivity achieved in detecting the cancer group was 62% (95% confidence interval = 0.50-0.74) at the index cutoff of 0.59. Fifty percent of stage I cancers had a DNA integrity index above this cutoff. All 11 patients with benign adnexal masses that clinically can be confused with malignant gynecological neoplasms demonstrated DNA integrity index < 0.59. Our findings suggest that increased DNA integrity in plasma DNA is associated with cancer, and measurement of DNA integrity may provide a simple and inexpensive measure for cancer detection.
ISSN:0008-5472
1538-7445