Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains

Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic m...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1992-10, Vol.37 (1), p.81-88
Main Authors: Hoh, Y.K, Tan, T.K, Yeoh, H.H
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ASPERGILLUS NIGER
Aspergillus niger - cytology
Aspergillus niger - enzymology
Aspergillus niger - physiology
beta-Glucosidase - isolation & purification
beta-Glucosidase - metabolism
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Biotechnology
Calcium - pharmacology
CITOLOGIA
CYTOLOGIE
CYTOLOGY
Enzyme engineering
ENZYMIC ACTIVITY
Fundamental and applied biological sciences. Psychology
FUSION DE PROTOPLASTES
FUSION DEL PROTOPLASTO
GLUCOSIDASA
GLUCOSIDASE
GLUCOSIDASES
HAPLOIDIA
HAPLOIDIE
HAPLOIDY
HETEROKARYOSIS
Improved methods for extraction and purification of enzymes
Kinetics
Methods. Procedures. Technologies
Polyethylene Glycols - pharmacology
PROTOPLAST FUSION
Protoplasts - physiology
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Summary:Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02788859