Loading…
Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains
Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic m...
Saved in:
| Published in: | Applied biochemistry and biotechnology 1992-10, Vol.37 (1), p.81-88 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863 |
| container_end_page | 88 |
| container_issue | 1 |
| container_start_page | 81 |
| container_title | Applied biochemistry and biotechnology |
| container_volume | 37 |
| creator | Hoh, Y.K Tan, T.K Yeoh, H.H |
| description | Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities. |
| doi_str_mv | 10.1007/BF02788859 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73485430</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16500852</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863</originalsourceid><addsrcrecordid>eNqFkM9LwzAcxYMoc04vHgWhB_EgVPOjaZPjHE4HAwXduaRpUiJdM_NtD_73ZnTo0dOD7_fD472H0CXB9wTj4uFxiWkhhODyCE0J5zLFVJJjNI1nllIq5Ck6A_jEmFDBiwmaRBUZ4VO0egu-97tWQZ_YAZzvEm-TyvQqbdpBe3C1ApPugq8H7bommcPOhMa17QBJ5xoTEuiDch2coxOrWjAXB52hzfLpY_GSrl-fV4v5OtVMFn2a51VluRVaYMUlrVnNOMNcC0MzbnJp60zGkFVOC4opzmtpKaGESSJNbUXOZuh29I2ZvgYDfbl1oE3bqs74AcqCZYJnDP8LkpxjLDiN4N0I6uABgrHlLritCt8lweV-4PJv4AhfH1yHamvqP3RcNP5vDn8FWrU2qE47-MWynNAC71tcjZhVvlRNiMjmXWYkdhfsB6z7iWo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16500852</pqid></control><display><type>article</type><title>Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains</title><source>Springer LINK Archives</source><creator>Hoh, Y.K ; Tan, T.K ; Yeoh, H.H</creator><creatorcontrib>Hoh, Y.K ; Tan, T.K ; Yeoh, H.H</creatorcontrib><description>Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/BF02788859</identifier><identifier>PMID: 1288415</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ASPERGILLUS NIGER ; Aspergillus niger - cytology ; Aspergillus niger - enzymology ; Aspergillus niger - physiology ; beta-Glucosidase - isolation & purification ; beta-Glucosidase - metabolism ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Biotechnology ; Calcium - pharmacology ; CITOLOGIA ; CYTOLOGIE ; CYTOLOGY ; Enzyme engineering ; ENZYMIC ACTIVITY ; Fundamental and applied biological sciences. Psychology ; FUSION DE PROTOPLASTES ; FUSION DEL PROTOPLASTO ; GLUCOSIDASA ; GLUCOSIDASE ; GLUCOSIDASES ; HAPLOIDIA ; HAPLOIDIE ; HAPLOIDY ; HETEROKARYOSIS ; Improved methods for extraction and purification of enzymes ; Kinetics ; Methods. Procedures. Technologies ; Polyethylene Glycols - pharmacology ; PROTOPLAST FUSION ; Protoplasts - physiology</subject><ispartof>Applied biochemistry and biotechnology, 1992-10, Vol.37 (1), p.81-88</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863</citedby><cites>FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4612706$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1288415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoh, Y.K</creatorcontrib><creatorcontrib>Tan, T.K</creatorcontrib><creatorcontrib>Yeoh, H.H</creatorcontrib><title>Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ASPERGILLUS NIGER</subject><subject>Aspergillus niger - cytology</subject><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - physiology</subject><subject>beta-Glucosidase - isolation & purification</subject><subject>beta-Glucosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Biotechnology</subject><subject>Calcium - pharmacology</subject><subject>CITOLOGIA</subject><subject>CYTOLOGIE</subject><subject>CYTOLOGY</subject><subject>Enzyme engineering</subject><subject>ENZYMIC ACTIVITY</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUSION DE PROTOPLASTES</subject><subject>FUSION DEL PROTOPLASTO</subject><subject>GLUCOSIDASA</subject><subject>GLUCOSIDASE</subject><subject>GLUCOSIDASES</subject><subject>HAPLOIDIA</subject><subject>HAPLOIDIE</subject><subject>HAPLOIDY</subject><subject>HETEROKARYOSIS</subject><subject>Improved methods for extraction and purification of enzymes</subject><subject>Kinetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>PROTOPLAST FUSION</subject><subject>Protoplasts - physiology</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFkM9LwzAcxYMoc04vHgWhB_EgVPOjaZPjHE4HAwXduaRpUiJdM_NtD_73ZnTo0dOD7_fD472H0CXB9wTj4uFxiWkhhODyCE0J5zLFVJJjNI1nllIq5Ck6A_jEmFDBiwmaRBUZ4VO0egu-97tWQZ_YAZzvEm-TyvQqbdpBe3C1ApPugq8H7bommcPOhMa17QBJ5xoTEuiDch2coxOrWjAXB52hzfLpY_GSrl-fV4v5OtVMFn2a51VluRVaYMUlrVnNOMNcC0MzbnJp60zGkFVOC4opzmtpKaGESSJNbUXOZuh29I2ZvgYDfbl1oE3bqs74AcqCZYJnDP8LkpxjLDiN4N0I6uABgrHlLritCt8lweV-4PJv4AhfH1yHamvqP3RcNP5vDn8FWrU2qE47-MWynNAC71tcjZhVvlRNiMjmXWYkdhfsB6z7iWo</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Hoh, Y.K</creator><creator>Tan, T.K</creator><creator>Yeoh, H.H</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19921001</creationdate><title>Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains</title><author>Hoh, Y.K ; Tan, T.K ; Yeoh, H.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ASPERGILLUS NIGER</topic><topic>Aspergillus niger - cytology</topic><topic>Aspergillus niger - enzymology</topic><topic>Aspergillus niger - physiology</topic><topic>beta-Glucosidase - isolation & purification</topic><topic>beta-Glucosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Biotechnology</topic><topic>Calcium - pharmacology</topic><topic>CITOLOGIA</topic><topic>CYTOLOGIE</topic><topic>CYTOLOGY</topic><topic>Enzyme engineering</topic><topic>ENZYMIC ACTIVITY</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUSION DE PROTOPLASTES</topic><topic>FUSION DEL PROTOPLASTO</topic><topic>GLUCOSIDASA</topic><topic>GLUCOSIDASE</topic><topic>GLUCOSIDASES</topic><topic>HAPLOIDIA</topic><topic>HAPLOIDIE</topic><topic>HAPLOIDY</topic><topic>HETEROKARYOSIS</topic><topic>Improved methods for extraction and purification of enzymes</topic><topic>Kinetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>PROTOPLAST FUSION</topic><topic>Protoplasts - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoh, Y.K</creatorcontrib><creatorcontrib>Tan, T.K</creatorcontrib><creatorcontrib>Yeoh, H.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoh, Y.K</au><au>Tan, T.K</au><au>Yeoh, H.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>37</volume><issue>1</issue><spage>81</spage><epage>88</epage><pages>81-88</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 x 10(-2) - 9.1 x 10(-2). After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and beta-glucosidase activities. One segregant showed a 2.5-fold increase in beta-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved beta-glucosidase activities.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>1288415</pmid><doi>10.1007/BF02788859</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 1992-10, Vol.37 (1), p.81-88 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73485430 |
| source | Springer LINK Archives |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ASPERGILLUS NIGER Aspergillus niger - cytology Aspergillus niger - enzymology Aspergillus niger - physiology beta-Glucosidase - isolation & purification beta-Glucosidase - metabolism Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Biotechnology Calcium - pharmacology CITOLOGIA CYTOLOGIE CYTOLOGY Enzyme engineering ENZYMIC ACTIVITY Fundamental and applied biological sciences. Psychology FUSION DE PROTOPLASTES FUSION DEL PROTOPLASTO GLUCOSIDASA GLUCOSIDASE GLUCOSIDASES HAPLOIDIA HAPLOIDIE HAPLOIDY HETEROKARYOSIS Improved methods for extraction and purification of enzymes Kinetics Methods. Procedures. Technologies Polyethylene Glycols - pharmacology PROTOPLAST FUSION Protoplasts - physiology |
| title | Protoplast fusion of beta-glucosidase-producing Aspergillus niger strains |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T11%3A26%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protoplast%20fusion%20of%20beta-glucosidase-producing%20Aspergillus%20niger%20strains&rft.jtitle=Applied%20biochemistry%20and%20biotechnology&rft.au=Hoh,%20Y.K&rft.date=1992-10-01&rft.volume=37&rft.issue=1&rft.spage=81&rft.epage=88&rft.pages=81-88&rft.issn=0273-2289&rft.eissn=1559-0291&rft.coden=ABIBDL&rft_id=info:doi/10.1007/BF02788859&rft_dat=%3Cproquest_cross%3E16500852%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c397t-66bbf5f8c80a592d3d35305c8e245e69fd49285b62720206d9f21213919edf863%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16500852&rft_id=info:pmid/1288415&rfr_iscdi=true |