Expression of estrogen receptor α and β genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle

• Published in: Neuroscience letters 2003-08, Vol.347 (2), p.131-135
• Main Authors: Hatoya, Shingo, Torii, Ryuzo, Kumagai, Daijiro, Sugiura, Kikuya, Kawate, Noritoshi, Tamada, Hiromichi, Sawada, Tsutomu, Inaba, Toshio
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Dog; Dogs; Estrogen Receptor alpha; Estrogen Receptor beta; Estrogen receptor α; Estrogen receptor β; Estrous Cycle; Female; Fundamental and applied biological sciences. Psychology; Hypothalamus; Hypothalamus - metabolism; Ovary; Ovary - metabolism; Pituitary; Pituitary Gland, Anterior - metabolism; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Steroid hormones. Cholecalciferol derivatives; Vertebrates: endocrinology
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(03)00639-6

Estrogen receptor α (ERα) and ERβ mRNA levels were measured in the mediobasal hypothalamus, the anterior pituitary and the ovary of beagle bitches at various stages of the estrous cycle. With polymerase chain reaction analysis we detected ERβ gene transcripts in all tissue samples. The levels of hypothalamic and pituitary ERα and β mRNAs increased from mid anestrus to proestrus and declined thereafter. In the ovary, ERα mRNA levels increased from proestrus to diestrus and were positively correlated with plasma progesterone levels ( r=0.62, P