Modification of Radiosensitivity by the So-called Tissue Recovery Stimulator. I. Radiosensitizing Effects of Solcoseryl

• Published in: JOURNAL OF RADIATION RESEARCH 1992, Vol.33 (4), p.282-289
• Main Authors: Kumar, A, Kimura, H, Aoyama, T, Sugahara, T
• Format: Article
• Language: English
• Subjects: Actihaemyl - pharmacology; Animals; Cells, Cultured - drug effects; Cells, Cultured - radiation effects; Cricetinae; Radiation Tolerance - drug effects; Radiation-Sensitizing Agents - pharmacology
• ISSN: 0449-3060; 1349-9157
• DOI: 10.1269/jrr.33.282

Radiosensitization/Gowth stimulation/Cell cycle distribution/V79 cells The effect of solcoseryl on the growth, radiosensitization and ability of V79 cells to recover from X-ray-induced damage has been observed. Solcoseryl at 0. 8 mg/ml was the optimal concentration for the stimulation of cell growth. Increased sensitivity to X-irradiation was found in the shoulder region of V79 cells treated before and after irradiation with solcoseryl (0. 8 mg/ml). The Dq and extrapolation number (n) decreased. Solcoseryl treatment apparently does not reduce split dose recovery or inhibit the repair of potentially lethal damage. Flow cytofluorometry studies of the cell cycle distribution and mitotic index show that solcoseryl inhibits the expression of radiation-induced cell arrest in the G2 phase of the cell cycle. Although this action increases radiation sensitization, additional mechanisms probably exist. Solcoseryl, a deproteinized dialysate of calf's blood stimulates respiratory enzymes1) and intracellular energy metabolism, thereby increasing the reserves of high energy phosphates2) and cellular regeneration and improving oxygen stimulation3). Solcoseryl also has been used in gastric ulcer therapy4). To our knowledge, however, there has been no report on radiosensitiza-tion produced by this growth stimulator. The experiments whose results are reported here were designed to measure the sensitivity to X-rays of V79 cells that had been exposed to solcoseryl before or after the irradiation. We examined the effects of solcoseryl on cell growth and survival characteristics, its effect on radiosensitization, and on the ability of V79 cells to recover from sublethal and potentially lethal damage.