The Effect of Lumbrokinase on P—selectin and E—selectin in Cerebral Ischemia Model of Rat

Purpose:To find the effect of lumbrokinase (LK) on P-selectin and E-selectin in ischemic rats. Methods:Male healthy Spragur-Dawley rats weighing 180-220 g(n=90) were divided into 4 groups: (1) normal control group (n=5),(2) sham-operated group (n=35), (3) ischemic group (n=35),(4) LK group (n=15).LK...

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Published in:Journal of traditional Chinese medicine 2003-06, Vol.23 (2), p.141-146
Main Authors: Zhang, Xiaoshu, Zhang, Jiatang, Kuang, Peizi, Lang, Senyang, Wu, Weiping, Yuan, Yumin, Liu, Jiexiao, Liu, Yu, Kuang, Peigen
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Ischemia - metabolism
Down-Regulation
E-Selectin - metabolism
Endopeptidases - pharmacology
E－选择素
Male
Medical sciences
Miscellaneous
P-Selectin - metabolism
P－选择素
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Rats
Rats, Sprague-Dawley
Tropical medicine
中医药疗法
脑缺血
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Summary:Purpose:To find the effect of lumbrokinase (LK) on P-selectin and E-selectin in ischemic rats. Methods:Male healthy Spragur-Dawley rats weighing 180-220 g(n=90) were divided into 4 groups: (1) normal control group (n=5),(2) sham-operated group (n=35), (3) ischemic group (n=35),(4) LK group (n=15).LK 10mg/kg (2000UK activity of LK) was given by intraperitoneal injection in the LK group 30 minutes before experiment. Same volume of normal saline was given in the sham-operated group and ischemic group. The ischemic model was made by modified Haruo Nagasawa's method. Immunohistochemistry was used to observe the P-selectin and E-selectin positive cells in the ischemic region. Results:P-selectin and E-selectin positive cells in ischemic regions were observed in the ischemic group, and the peak of expression was at 6 hours and 12 hours, respectively. The similar changes were not observed in normal control group. There were only a few positive cells in the sham-operated group. In LK group, the P-selectin and E-selectin positive cells were significantly less than those in the ischemic group (P
ISSN:0255-2922