Electron spin resonance study on the permeability of superoxide radicals in lipid bilayers and biological membranes

The permeability of lipid bilayers and biological membranes to superoxide free radicals was examined by using superoxide dismutase (SOD)-loaded lipid vesicles and SOD-loaded erythrocyte ghosts. After exposing SOD lipid vesicles and SOD ghosts to enzymatically produced superoxide radicals and using s...

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Bibliographic Details
Published in:FEBS letters 1992-07, Vol.305 (3), p.233-236
Main Authors: Mao, Guo Dong, Poznansky, Mark J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological membranes
Cell Membrane Permeability - physiology
E.S.R
Electron spin resonance
Electron Spin Resonance Spectroscopy
Erythrocyte ghost
Erythrocyte Membrane - metabolism
erythrocytes
Free Radical Scavengers
free radicals
Free Radicals - metabolism
Fundamental and applied biological sciences. Psychology
ghosts
Humans
In Vitro Techniques
lipid bilayers
Lipid Bilayers - metabolism
man
Membrane physicochemistry
Molecular biophysics
Permeability
Permeability, Lipid vesicle
Superoxide Dismutase - metabolism
Superoxide radical
Superoxides - metabolism
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Summary:The permeability of lipid bilayers and biological membranes to superoxide free radicals was examined by using superoxide dismutase (SOD)-loaded lipid vesicles and SOD-loaded erythrocyte ghosts. After exposing SOD lipid vesicles and SOD ghosts to enzymatically produced superoxide radicals and using spin-trapping and electron spin resonance (ESR) techniques, we found that SOD entrapped within erythrocyte ghosts effectively scavenges external O .− 2 while SOD inside the lipid bilayers has no effect. These results confirm that O .− 2 is able to cross through a biological plasma membrane but not across a pure lipid bilayer. The data provide instruction as to how and where anti-oxidant therapy is to be approached relative to the site of oxygen free radical production.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80675-7