Determination of dopamine in single rat pheochromocytoma cell by capillary electrophoresis with amperometric detection

A method is described for the direct identification of dopamine in single cultured rat pheochromocytoma cell by capillary electrophoresis (CE) with amperometric detection. The separation and detection conditions were optimized. The dopamine in single cell analysis was identified based on the migrati...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-07, Vol.792 (2), p.381-385
Main Authors: Zhang, Liyao, Qv, Sanfu, Wang, Zongli, Cheng, Jieke
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Dopamine
Dopamine - analysis
Electrochemistry - methods
Electrophoresis, Capillary - methods
Fundamental and applied biological sciences. Psychology
PC12 Cells
Pheochromocytoma - chemistry
Rats
Reference Standards
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method is described for the direct identification of dopamine in single cultured rat pheochromocytoma cell by capillary electrophoresis (CE) with amperometric detection. The separation and detection conditions were optimized. The dopamine in single cell analysis was identified based on the migration time of standard dopamine and internal standard (epinephrine). The amount of dopamine in a single cell ranged from 0.29 to 1.28 fmol.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00323-4