Brain expression of apurinic/apyrimidinic endonuclease (APE/Ref-1) multifunctional DNA repair enzyme gene in the mouse with special reference to the suprachiasmatic nucleus

Multifunctional mammalian apurinic/apyrimidinic endonuclease (APE, also known as redox factor-1; Ref-1) repairs baseless sites of damaged DNA caused by oxidative stress and regulates the redox state of various DNA binding proteins. Here, we examined the expression of APE/Ref-1 m-RNA in the mouse bra...

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Bibliographic Details
Published in:Neuroscience research 2003-08, Vol.46 (4), p.443-452
Main Authors: Kimura, Hidehito, Dong, Xing, Yagita, Kazuhiro, Okamura, Hitoshi
Format: Article
Language:English
Subjects:
Animals
Apurinic/apyrimidinic endonuclease
Autoradiography - methods
Brain
Brain - anatomy & histology
Brain - metabolism
Brain Mapping
Carbon-Oxygen Lyases - genetics
Carbon-Oxygen Lyases - metabolism
Circadian
Circadian Rhythm - physiology
DNA-(Apurinic or Apyrimidinic Site) Lyase
Gene Expression
Immunohistochemistry
In Situ Hybridization
Light
Mice
Mice, Inbred BALB C
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Spinal Cord - metabolism
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Time Factors
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Summary:Multifunctional mammalian apurinic/apyrimidinic endonuclease (APE, also known as redox factor-1; Ref-1) repairs baseless sites of damaged DNA caused by oxidative stress and regulates the redox state of various DNA binding proteins. Here, we examined the expression of APE/Ref-1 m-RNA in the mouse brain by in situ hybridization. We detected APE/Ref-1 transcripts throughout the mouse brain particularly in the clock oscillating neurons of the suprachiasmatic nucleus (SCN), hippocampal pyramidal cells, granular cells, and in monoaminergic neurons. In the circadian center SCN, levels of APE/ref-1 mRNA transcripts were constantly high, and were not influenced by either circadian rhythms or by exposure to light.
ISSN:0168-0102
1872-8111
DOI:10.1016/S0168-0102(03)00124-X