Sesquiterpene lactone from Wunderlichia crulsiana inhibits the respiratory burst of leukocytes triggered by distinct biochemical pathways

The sesquiterpene lactone tubiferin was chemically purified from the brazilian native plant Wunderlichia crulsiana and identified by NMR and GC/MS data. Its ability to inhibit the respiratory burst of peritoneal inflammatory polymorphonuclear leukocytes (PMN) stimulated upon addition of phorbol miri...

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Bibliographic Details
Published in:Life sciences (1973) 2003-09, Vol.73 (17), p.2161-2169
Main Authors: Nuñez, Cecilia Veronica, Zacheu, Fabı́ola Maria, Pinto, Ernani, Roque, Nı́dia Franca, Colepicolo, Pio, Brigagão, Maı́sa Ribeiro Pereira Lima
Format: Article
Language:English
Subjects:
Animals
Anti-inflammatory
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Asteraceae - chemistry
Brazil
Dose-Response Relationship, Drug
Edema - chemically induced
Edema - drug therapy
Edema - pathology
Hindlimb
Indomethacin - pharmacology
Lactones - pharmacology
Male
Mice
Neutrophils - drug effects
Neutrophils - metabolism
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polymorphonuclear leukocyte
Respiratory Burst - drug effects
Sesquiterpene lactone
Sesquiterpenes - isolation & purification
Sesquiterpenes - pharmacology
Singlet Oxygen - analysis
Superoxide anion
Tubiferin 13C NMR data
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Summary:The sesquiterpene lactone tubiferin was chemically purified from the brazilian native plant Wunderlichia crulsiana and identified by NMR and GC/MS data. Its ability to inhibit the respiratory burst of peritoneal inflammatory polymorphonuclear leukocytes (PMN) stimulated upon addition of phorbol miristate acetate (PMA), opsonized zymosan (OZ), and N-formyl-methionyl-leucyl-phenylalanine (fMLP) was evaluated. The tubiferin inhibition was more pronounced when PMN were stimulated through the protein kinase C pathway (PMA) compared to the alternative complement pathway (OZ). The inhibition when PMN were triggered by a chemoatractant stimulus (fMLP) was similar to that achieved with OZ-stimulated phagocytes. Tubiferin showed dose-dependent effects on the PMN respiratory burst triggered by the three different substances, and also decreased substancially the carrageenan-induced mice paw edema.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(03)00600-3