Role of T cells in the adjuvant effect of Bacillus firmus on the immune system of mice: Intranasal and intratracheal immunization study with ovalbumin

• Published in: Folia microbiologica 2003, Vol.48 (3), p.427-434
• Main Authors: MLCKOVA, P, CECHOVA, D, MARUSKOVA, L, CHALUPNA, P, NOVOTNA, O, PROKESOVA, L
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - pharmacology; Administration, Intranasal; Animals; Bacillus - immunology; Bacillus firmus; Biological and medical sciences; Cytokines - immunology; Cytokines - metabolism; Female; Fundamental and applied biological sciences. Psychology; Immunization - methods; Immunization - standards; Immunoglobulin G - blood; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; Microbiology; ovalbumin; Ovalbumin - administration & dosage; Ovalbumin - immunology; T-Lymphocytes - immunology
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/BF02931379

Functions of T cells were determined after intranasal and intratracheal immunization of mice with ovalbumin (Ova) and Bacillus firmus (Bf), a Gram-positive nonpathogenic bacterium of the external environment, or delipidated Bf (dBf) as adjuvants, with the aim to elucidate the mechanism of support of Ova-specific antibody production caused by Bf that had been observed in an identical experiment. Neither Bf nor dBf in a mixture with Ova stimulated Ova-specific T-cell response tested as antigen-specific blast transformation. By contrast, a mild polyclonal stimulation was observed in splenocytes from mice given dBf. In vitro incubation of splenocytes with 100 micrograms (but not 10 micrograms) of Bf or dBf led to a highly significant inhibition of proliferation below the control level in all groups of animals. Supernatants of splenocyte cultures were further tested for cytokine production. IL-10 and IFN-gamma were released after in vitro challenge with dBf and in some cases also with Bf. Analysis of sera demonstrated that administration of Ova + adjuvant brought about an increase in anti-Ova IgG1, IgG2a and IgG2b whereas treatment with Ova alone caused a rise in IgG1 only. The role of Bf or dBf in the enhancement of antigen-specific antibody production could be in influencing macrophages and inducing cytokine milieu composed of IL-10, IFN-gamma and other factors that leads to a bystander stimulation of specifically activated Ova-B cell receptor (Ova-BCR)-bearing cells.