Fractionation of grape tannins and analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene–divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecul...

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Bibliographic Details
Published in:Phytochemical analysis 2003-07, Vol.14 (4), p.202-208
Main Authors: Perret, Camille, Pezet, Roger, Tabacchi, Raphael
Format: Article
Language:English
Subjects:
Anthocyanins - analysis
Biflavonoids
Catechin - analysis
degree of polymerisation
fractionation
grape
Isomerism
MALDI-TOF/MS
Molecular Structure
Plant Extracts - chemistry
Proanthocyanidins
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectrum Analysis
tannins
Tannins - analysis
Vitis - chemistry
Vitis vinifera
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Summary:Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene–divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecular weight tannins. The mean mDP of each collected fraction was determined by acid‐catalysed degradation in the presence of a nucleophilic reagent. The fractionation method produced a linear gradient of mDP varying between 1.84 and 19.34. The fractions were partially characterised by matrix assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF/MS). The spectra showed a complex mixture of proanthocyanidins and galloylated proanthocyanidins up to 4000 amu. Copyright © 2003 John Wiley & Sons, Ltd.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.705