Expression of the Wdr9 gene and protein products during mouse development

Human WDR9 has been mapped to chromosome 21, within one of the Down syndrome (DS) critical regions. Here, we study the expression pattern of the murine Wdr9 gene and its protein product. We show that Wdr9 is broadly expressed in the mouse embryo by means of in situ hybridization and immunohistochemi...

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Bibliographic Details
Published in:Developmental dynamics 2003-08, Vol.227 (4), p.608-614
Main Authors: Huang, He, Rambaldi, Isabel, Daniels, Eugene, Featherstone, Mark
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
bromodomain
Chromatin Assembly and Disassembly - genetics
DNA Helicases
Down syndrome
Embryo, Mammalian - metabolism
Gene Expression Regulation, Developmental
Immunohistochemistry
In Situ Hybridization
Luciferases
Mice
mouse development
NIH 3T3 Cells
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Precipitin Tests
transcription
Transcription Factors - genetics
Transcriptional Activation - genetics
Transfection
WD repeats
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Summary:Human WDR9 has been mapped to chromosome 21, within one of the Down syndrome (DS) critical regions. Here, we study the expression pattern of the murine Wdr9 gene and its protein product. We show that Wdr9 is broadly expressed in the mouse embryo by means of in situ hybridization and immunohistochemistry. Wdr9 expression levels are dynamic during embryonic development as revealed by Northern blot analysis. We further show that WDR9 is a nuclear protein associated with BRG1, a SWI/SNF complex component. We also demonstrate that a polyglutamine‐containing region of the protein functions as a transcriptional activation domain. We propose that WDR9 is a transcriptional regulator involved in chromatin remodeling through the action of two bromodomains and contacts to the SWI/SNF complex. These results may provide a molecular basis for the association of WDR9 with DS. Developmental Dynamics 227:608–614, 2003. © 2003 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.10344