New EPR method for cellular surface characterization

An electron paramagnetic resonance (EPR)-based membrane surface characterization method is presented to detect the properties of the carbohydrate-rich part of membrane surfaces as well as carbohydrate interaction with other membrane constituents and water-soluble molecules. The proposed method relie...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of membrane biology 2003-05, Vol.193 (1), p.15-22
Main Authors: Strancar, J, Schara, M, Pecar, S
Format: Article
Language:English
Subjects:
Algorithms
Animals
Carbohydrates
Carbohydrates - chemistry
Cattle
Cell Membrane - chemistry
Electron paramagnetic resonance
Electron spin resonance
Electron Spin Resonance Spectroscopy - methods
Erythrocyte Membrane - chemistry
Gangliosides - chemistry
Glycocalyx - chemistry
Glycolipids
Heterogeneity
Lipid bilayers
Lipid Bilayers - chemistry
Lipids
Macromolecular Substances
Membrane Fluidity
Membrane Microdomains - chemistry
Membranes
Optimization
Spin Labels
Sucrose
Sucrose - chemistry
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An electron paramagnetic resonance (EPR)-based membrane surface characterization method is presented to detect the properties of the carbohydrate-rich part of membrane surfaces as well as carbohydrate interaction with other membrane constituents and water-soluble molecules. The proposed method relies on the spin-labeling and spectral decomposition based on spectral simulation and optimization with EPRSIM software. In order to increase the sensitivity of characterization to the carbohydrate-rich part of the membrane surface, the sucrose-contrasting approach is introduced. With this method, which was established on model membranes with glycolipids and tested on erythrocyte membrane, we were able to characterize the surface and lipid bilayer lateral heterogeneity. Additionally, some properties of the interaction between glycocalyx and lipid bilayer as well as between glycocalyx and sucrose molecules were determined. The experiments also provided some information about the anchoring and aggregation of the glycosylated molecules. According to the results, some functions of the glycosylated surface are discussed.
ISSN:0022-2631
1432-1424
DOI:10.1007/s00232-002-2003-7