Trypanosoma brucei: Trypanosome strain typing using PCR analysis of mobile genetic elements (MGE-PCR)

We describe the development of a single-primer amplification system, which uses the trypanosomal mobile genetic element RIME as a molecular marker for the differentiation of Trypanosoma brucei stocks. Using a well-characterised set of T. brucei stocks from southeast Uganda, Kenya and Zambia, we have...

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Bibliographic Details
Published in:Experimental parasitology 2003-05, Vol.104 (1), p.26-32
Main Authors: Tilley, A, Welburn, S.C, Fèvre, E.M, Feil, E.J, Hide, G
Format: Article
Language:English
Subjects:
Animals
Cattle
Cluster Analysis
DNA, Protozoan - analysis
Genetic Markers - genetics
Human-infectivity
Humans
Interspersed Repetitive Sequences - genetics
Mice
Mobile genetic element
Molecular epidemiology
PCR
Phylogeny
Polymerase Chain Reaction - methods
Serum resistance associated ( SRA) gene
Sleeping sickness
Swine
Trypanosoma brucei brucei
Trypanosoma brucei brucei - classification
Trypanosoma brucei brucei - genetics
Trypanosoma brucei rhodesiense
Trypanosomiasis, African - parasitology
Tsetse Flies
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Summary:We describe the development of a single-primer amplification system, which uses the trypanosomal mobile genetic element RIME as a molecular marker for the differentiation of Trypanosoma brucei stocks. Using a well-characterised set of T. brucei stocks from southeast Uganda, Kenya and Zambia, we have evaluated the application of this technique, termed MGE-PCR (mobile genetic element PCR) for the typing of trypanosome strains. The technique revealed considerable variation between stocks and was sufficiently specific to amplify trypanosomal DNA in the presence of host DNA. The results showed a clear distinction between human-infective and non-human-infective stocks. Comparative studies on these stocks using markers for the human serum resistance associated ( SRA) gene, which identifies human-infective stocks, demonstrated complete agreement between MGE-PCR derived groups and human-infectivity status. Furthermore, MGE-PCR detects high levels of variability within the T. b. brucei and T. b. rhodesiense groups and is therefore a powerful discriminatory tool for tracking individual T. brucei genotypes and strains.
ISSN:0014-4894
1090-2449
DOI:10.1016/S0014-4894(03)00114-0