Characterization of the species- and stage-specificity of two monoclonal antibodies against Leishmania amazonensis

Leishmania metacyclogenesis is associated with changes in morphology, gene expression, and structural alterations of the lipophosphoglycan (LPG), the promastigote most abundant surface glycolipid. Purification of metacyclics is accomplished using lectins or monoclonal antibodies (MAbs) that exploit...

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Bibliographic Details
Published in:Experimental parasitology 2003-03, Vol.103 (3), p.152-159
Main Authors: Chaves, C.S., Soares, D.C., Da Silva, R.P., Saraiva, E.M.
Format: Article
Language:English
Subjects:
Agglutination Tests
Animals
Antibodies, Monoclonal - biosynthesis
Antibodies, Monoclonal - immunology
Antibodies, Protozoan - biosynthesis
Antibodies, Protozoan - immunology
Antibody Specificity
Biological and medical sciences
Diagnostic
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Glycosphingolipids - immunology
Human protozoal diseases
Humans
Immunoblotting
Infectious diseases
Leishmania
Leishmania - classification
Leishmania - growth & development
Leishmania - immunology
Leshmaniasis
Life Cycle Stages
Medical sciences
Mice
Mice, Inbred BALB C
Monoclonal antibodies
Parasitic diseases
Protozoa
Protozoal diseases
Species Specificity
Systematics. Geographical distribution. Morphology. Cytology
Taxonomy
Trypanosomatina - classification
Trypanosomatina - immunology
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Summary:Leishmania metacyclogenesis is associated with changes in morphology, gene expression, and structural alterations of the lipophosphoglycan (LPG), the promastigote most abundant surface glycolipid. Purification of metacyclics is accomplished using lectins or monoclonal antibodies (MAbs) that exploit stage-specific differences in the LPG. Besides, LPG displays extensive interspecies polymorphisms and is synthesized by promastigotes of all species investigated to date. In this work we studied the species- and stage-specificity of two MAbs (3A1-La and LuCa-D5) used to purify metacyclics of Leishmania amazonensis. Their ability to recognize different members of the Trypanosomatidae family was tested by direct agglutination, indirect immunofluorescence, and dot-blot analysis of LPG. We found that both MAbs were highly selective for L. amazonensis: 3A1-La recognized only promastigotes and LuCa-D5 labeled amastigote and promastigote stages of this species. These MAbs might be useful for Leishmania typing.
ISSN:0014-4894
1090-2449
DOI:10.1016/S0014-4894(03)00098-5