Evaluation of a Polymerase Chain Reaction Amplification Method for Mycobacterium tuberculosis Complex on Samples from Different Sources

Using primers specific for the IS6110 region of Mycobacterium tuberculosis complex, successful amplification by the polymerase chain reaction was demonstrated in 81 of 84 archive specimens from patients who had been clinically diagnosed 2 months to 16 y previously as having tuberculosis. Depending o...

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Bibliographic Details
Published in:Scandinavian journal of infectious diseases 2003-01, Vol.35 (5), p.329-331
Main Authors: Cataloluk, Osman, Karsligil, Tekin, Bayazit, Nurhayat
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
DNA, Bacterial - analysis
Female
Fundamental and applied biological sciences. Psychology
Humans
Male
Microbiology
Molecular Sequence Data
Mycobacterium tuberculosis - isolation & purification
Polymerase Chain Reaction - methods
Sampling Studies
Sensitivity and Specificity
Specimen Handling
Turkey
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Summary:Using primers specific for the IS6110 region of Mycobacterium tuberculosis complex, successful amplification by the polymerase chain reaction was demonstrated in 81 of 84 archive specimens from patients who had been clinically diagnosed 2 months to 16 y previously as having tuberculosis. Depending on the time of storage of the specimens, extra DNA bands were found in addition to the IS6110 region band.
ISSN:0036-5548
1651-1980
DOI:10.1080/00365540310005404