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A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor

Monoclonal antibodies to specific families of TCR variable domains serve as highly useful immunochemical tools for basic research in T-cell biology and diagnosis of autoimmune diseases. Monoclonal antibody MEM-262 characterized in this communication recognizes beta chains of the TCR expressed by HPB...

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Bibliographic Details
Published in:Immunology letters 2003-08, Vol.88 (2), p.105-108
Main Authors: Pavlistová, Dagmar, Drbal, Karel, Hilgert, Ivan, Horejsí, Václav
Format: Article
Language:English
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Summary:Monoclonal antibodies to specific families of TCR variable domains serve as highly useful immunochemical tools for basic research in T-cell biology and diagnosis of autoimmune diseases. Monoclonal antibody MEM-262 characterized in this communication recognizes beta chains of the TCR expressed by HPB-ALL cell line (carrying Vbeta5.3) and a small subset of peripheral blood T cells. This subset is larger than that recognized by a previously described Vbeta5.3-specific mAb. MEM-262 potently stimulates selective expansion of the T-cell subset, efficiently immunoisolates native TCR complexes as well as free beta chains and uniquely recognizes denatured TCRbeta chains under the conditions of Western blotting.
ISSN:0165-2478