Attenuated total reflectance Fourier transform mid-infrared spectroscopic quantification of sorbitol and sorbose during a Gluconobacter biotransformation process

Mid-infrared spectroscopy (MIRS) was used to simultaneously detect and predict concentrations of D-sorbitol and L-sorbose during a Gluconobacter suboxydans biotransformation. Quantitative models for both these compounds were developed for the entire time-course of the process and validated externall...

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Bibliographic Details
Published in:Biotechnology letters 2003-02, Vol.25 (3), p.257-260
Main Authors: MACAULEY-PATRICK, Sue, ARNOLD, S. Alison, MCCARTHY, Brendan, HARVEY, Linda M, MCNEIL, Brian
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactors - microbiology
Biotransformation
Correlation coefficient
Fourier transforms
Fundamental and applied biological sciences. Psychology
Gluconobacter - chemistry
Gluconobacter - metabolism
Infrared spectroscopy
Sorbitol - analysis
Sorbitol - chemistry
Sorbitol - metabolism
Sorbose - analysis
Sorbose - biosynthesis
Sorbose - chemistry
Spectroscopy, Fourier Transform Infrared - methods
Spectrum analysis
Studies
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Summary:Mid-infrared spectroscopy (MIRS) was used to simultaneously detect and predict concentrations of D-sorbitol and L-sorbose during a Gluconobacter suboxydans biotransformation. Quantitative models for both these compounds were developed for the entire time-course of the process and validated externally using samples not included in the original modelling exercise, giving standard errors of prediction of 3.29 and 3.3% for sorbitol and sorbose, respectively, and a correlation coefficient close to 1.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1022351001284