Characterization of DNA sequences that mediate nuclear protein binding to the regulatory region of the Pisum sativum (pea) chlorophyl a/b binding protein gene AB80: identification of a repeated heptamer motif

• Published in: The Plant journal : for cell and molecular biology 1992-05, Vol.2 (3), p.301-309
• Main Authors: Argüello, Gerardo, García‐Hernández, Enrique, Sánchez, Mireya, Gariglio, Patricio, Herrera‐Estrella, Luis, Simpson, June
• Format: Article
• Language: English
• Subjects: Base Sequence; Binding Sites; Chlorophyll - metabolism; DNA - genetics; DNA-Binding Proteins - metabolism; Fabaceae - genetics; Fabaceae - metabolism; Fungal Proteins - metabolism; Genes, Plant; Genes, Regulator; Molecular Sequence Data; Nuclear Proteins - metabolism; Plant Proteins - metabolism; Plants - genetics; Plants - metabolism; Plants, Medicinal; Protein Binding; Repetitive Sequences, Nucleic Acid; Saccharomyces cerevisiae Proteins; Sequence Homology, Nucleic Acid; Species Specificity; Transcription Factors
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1992.t01-30-00999.x

Summary Two protein factors binding to the regulatory region of the pea chlorophyl a/b binding protein gene AB80 have been identified. One of these factors is found only in green tissue but not in etiolated or root tissue. The second factor (denominated ABF‐2) binds to a DNA sequence element that contains a direct heptamer repeat TCTCAAA. It was found that presence of both of the repeats is essential for binding. ABF‐2 is present in both green and etiolated tissue and in roots and factors analogous to ABF‐2 are present in several plant species. Computer analysis showed that the TCTCAAA motif is present in the regulatory region of several plant genes.