Late differentiation contributes to the apparent increase in sensory neuron number in juvenile rat

Using both profile counts and unbiased stereological methods, estimates of neuron number in the lumbar dorsal root ganglia of the rat have been shown to increases approximately 35% during postnatal life [J. Comp. Neurol. 386 (1997) 8–15; J. Comp. Neurol. 449 (2002) 158–165]. The mechanism underlying...

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Bibliographic Details
Published in:Brain research. Developmental brain research 2003-08, Vol.144 (1), p.91-98
Main Author: Farel, Paul B.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Axons - metabolism
Axons - ultrastructure
Bromodeoxyuridine - pharmacokinetics
Cell Count - methods
Dorsal root ganglion
Ganglia, Spinal - growth & development
Ganglia, Spinal - ultrastructure
Horseradish Peroxidase - pharmacokinetics
Immunohistochemistry - methods
In Vitro Techniques
Microscopy, Electron - methods
Neurofilament Proteins - metabolism
Neurons, Afferent - classification
Neurons, Afferent - physiology
Neurons, Afferent - ultrastructure
Postnatal differentiation
Radiation-Sensitizing Agents
Rat
Rats
Rats, Sprague-Dawley
Sural Nerve - growth & development
Sural Nerve - metabolism
Sural Nerve - ultrastructure
Time Factors
Tubulin - metabolism
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Summary:Using both profile counts and unbiased stereological methods, estimates of neuron number in the lumbar dorsal root ganglia of the rat have been shown to increases approximately 35% during postnatal life [J. Comp. Neurol. 386 (1997) 8–15; J. Comp. Neurol. 449 (2002) 158–165]. The mechanism underlying this addition of neurons was investigated. No evidence of incorporation of (BrdU), a mitotic marker, was found. Similarly, counts of myelinated and unmyelinated axons in the sural nerve were the same in neonates and adults. These results are not consistent with the possibility that neurogenesis accounts for neuron addition. A population of neurons that stains with TuJ1, an antibody against neuronal class III β tubulin, but not with an antibody against the phosphorylated and non-phosphorylated forms of heavy chain neurofilament protein (NF-H) was found in neonates, but not adults. These less-differentiated (type-L) neurons are not detected by either profile counts or unbiased stereology and do not transport HRP retrogradely. Maturation of this pool of incompletely differentiated neurons appears to be one mechanism whereby neuron number is augmented during postnatal life.
ISSN:0165-3806
DOI:10.1016/S0165-3806(03)00163-9