Adsorptive stripping voltammetric determination of cefetamet in human urine

• Published in: Journal of pharmaceutical and biomedical analysis 2003-08, Vol.32 (4), p.957-966
• Main Authors: ALEKSIC, Mara, MILOVANOVIC, Ljiljana, KAPETANOVIC, Vera
• Format: Article
• Language: English
• Subjects: Adsorption; Adsorptive stripping voltammetry; Analysis; Biological and medical sciences; Cefetamet; Ceftizoxime - analogs & derivatives; Ceftizoxime - chemistry; Ceftizoxime - urine; Electrochemistry; General pharmacology; Humans; Medical sciences; Pharmacology. Drug treatments; Polarography - methods; Technology, Pharmaceutical - methods; Urine determination
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(03)00197-3

On the basis of previously established mechanism of cefetamet (CEF) reduction, two methods were suggested for CEF determination—differential pulse polarographic and differential pulse adsorptive stripping voltammetric method. Two pH values were chosen, 2.0 and 8.4, where the electrochemical process was defined as one four-electron and two two-electron processes, respectively. The methods were performed in Britton–Robinson (BR) buffer and the corresponding calibration graphs were constructed and statistical parameters were evaluated. Applying the AdSV method at pH 2.0 linearity was achieved from 2×10 −8 to 2×10 −7 M with limit detection and limit determination of 4×10 −9 and 1.4×10 −8 M, respectively. At pH 8.4, the linearity was obtained between 6×10 −8 and 6×10 −7 M, with limit detection and limit determination of 1.5×10 −8 and 5×10 −8 M, respectively. Since the AdSV method enabled lower concentrations of CEF to be determined, this method was tested for CEF determination in spiked urine samples, and DPP method was used as a comparative one.